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Literature summary for 2.5.1.46 extracted from

  • Nguyen, S.; Jones, D.C.; Wyllie, S.; Fairlamb, A.H.; Phillips, M.A.
    Allosteric activation of trypanosomatid deoxyhypusine synthase by a catalytically dead paralog (2013), J. Biol. Chem., 288, 15256-15267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.46

Cloned(Commentary)

Cloned (Comment) Organism
gene Tb10.70.4900, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3), coexpression with gene TB927.1.870 results in heterotetramer formation with a 3000fold increase in enzyme activity Trypanosoma brucei brucei
gene TB927.1.870, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3), coexpression with gene Tb10.70.4900 in Escherichia coli strain BL21 (DE3) results in heterotetramer formation with a 3000fold increase in enzyme activity Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information generation of a knockout mutant by gene replacement Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
N1-guanyl-1,7-diaminoheptane GC7, inhibits the activity of TbDHSc-TbDBHSp heterotetrameric complex; GC7, inhibits the activity of TbDHSc-TbDBHSp heterotetrameric complex Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 [eIF5A-precursor]-lysine pH 7.5, 37°C, heterotetrameric DHS Trypanosoma brucei brucei
0.043
-
 spermidine pH 7.5, 37°C, heterotetrameric DHS Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast
-
 Trypanosoma brucei brucei 20023
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine Trypanosoma brucei brucei
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[eIF5A-precursor]-lysine + spermidine Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q38BX0 gene Tb10.70.4900
-
Trypanosoma brucei brucei Q4GZD1 gene TB927.1.870
-
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 Q38BX0 gene Tb10.70.4900
-
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 Q4GZD1 gene TB927.1.870
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Trypanosoma brucei brucei
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity cromatography and gel filtration Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine
-
 Trypanosoma brucei brucei [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[eIF5A-precursor]-lysine + spermidine
-
 Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Synonyms

Synonyms Comment Organism
DHS
-
 Trypanosoma brucei brucei
TbDHSc
-
 Trypanosoma brucei brucei
TbDHSp
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 spermidine pH 7.5, 37°C, heterotetrameric DHS Trypanosoma brucei brucei
0.018
-
 [eIF5A-precursor]-lysine pH 7.5, 37°C, heterotetrameric DHS Trypanosoma brucei brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trypanosoma brucei brucei

Cofactor

Cofactor Comment Organism Structure
NAD+ required, Km value is 0.082 mM, kcat is 0.018/s for the heterotetrameric DHS Trypanosoma brucei brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0015
-
 pH 7.5, 37°C, heterotetrameric DHS Trypanosoma brucei brucei N1-guanyl-1,7-diaminoheptane

General Information

General Information Comment Organism
additional information Trypanosoma brucei encodes two deoxyhypusine synthase isozymes TbDHSc and TbDHSp that form a functional complex Trypanosoma brucei brucei
additional information Trypanosoma brucei encodes two deoxyhypusine synthase isozymes TbDHSc and TbDHSp that form a functional heterotetrameric complex Trypanosoma brucei brucei
physiological function the enzyme deoxyhypusine synthase is required for spermidine-dependent hypusine modification of a lysine residue in the essential translation factor eIF5A. Enzyme DHS is essential for infectivity of Trypanosoma brucei in mice Trypanosoma brucei brucei