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Literature summary for 2.5.1.46 extracted from

  • Quintas-Granados, L.I.; Carvajal Gamez, B.I.; Villalpando, J.L.; Ortega-Lopez, J.; Arroyo, R.; Azuara-Liceaga, E.; Alvarez-Sanchez, M.E.
    Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis (2016), Biochimie, 123, 37-51.
    View publication on PubMed
Search for more literature for 2.5.1.46

Cloned(Commentary)

Cloned (Comment) Organism
gene TvDHS, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with recombinant TveIF-5A (rTveIF-5A) precursor protein and formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis

Protein Variants

Protein Variants Comment Organism
D314A site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase Trichomonas vaginalis
H313A site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase Trichomonas vaginalis

Inhibitors

Inhibitors Comment Organism Structure
N''-guanyl-1,7-diaminoheptane GC7, a spermidine analogue Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 spermidine pH 9.0, 37°C, recombinant enzyme Trichomonas vaginalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 4 * 43000, recombinant enzyme, SDS-PAGE Trichomonas vaginalis
170000
-
 recombinant enzyme, native PAGE Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichomonas vaginalis the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 ?
-
 ?
additional information Trichomonas vaginalis CNCD 147 the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 ?
-
 ?
[TveIF-5A-precursor]-lysine + spermidine Trichomonas vaginalis
-
 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[TveIF-5A-precursor]-lysine + spermidine Trichomonas vaginalis CNCD 147
-
 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis F1AQF9 gene TvDHS
-
Trichomonas vaginalis CNCD 147 F1AQF9 gene TvDHS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 Trichomonas vaginalis ?
-
 ?
additional information formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis ?
-
 ?
additional information the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 Trichomonas vaginalis CNCD 147 ?
-
 ?
additional information formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis CNCD 147 ?
-
 ?
[TveIF-5A-precursor]-lysine + spermidine
-
 Trichomonas vaginalis [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[TveIF-5A-precursor]-lysine + spermidine transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue Trichomonas vaginalis [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[TveIF-5A-precursor]-lysine + spermidine
-
 Trichomonas vaginalis CNCD 147 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[TveIF-5A-precursor]-lysine + spermidine transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue Trichomonas vaginalis CNCD 147 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, recombinant enzyme, SDS-PAGE Trichomonas vaginalis

Synonyms

Synonyms Comment Organism
DHS
-
 Trichomonas vaginalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trichomonas vaginalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Trichomonas vaginalis