Cloned (Comment) | Organism |
---|---|
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli | Ipomoea neei |
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli | Ipomoea hederifolia |
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli | Ipomoea alba |
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli | Ipomoea meyeri |
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli | Distimake quinquefolius |
Protein Variants | Comment | Organism |
---|---|---|
I277D | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and slightly reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
I277D/N281D | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
N266H | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
N266H/I277D | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine, but unaltered deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
N266H/I277D/N281D | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
N266H/N281D | site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
N281D | site-directed mutagenesis, the mutant shows slightly reduced HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type | Ipomoea neei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41300 | - |
- |
Distimake quinquefolius |
41500 | - |
- |
Ipomoea alba |
41500 | - |
- |
Ipomoea meyeri |
41600 | - |
x * 41600, about, sequence calculation | Ipomoea neei |
41600 | - |
x * 41600, about, sequence calculation | Ipomoea hederifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + spermidine | Ipomoea neei | - |
sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | Ipomoea hederifolia | - |
sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | Ipomoea alba | - |
sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | Ipomoea meyeri | - |
sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | Distimake quinquefolius | - |
sym-homospermidine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Distimake quinquefolius | - |
gene hss | - |
Ipomoea alba | - |
gene hss | - |
Ipomoea hederifolia | - |
gene hss | - |
Ipomoea meyeri | - |
gene hss | - |
Ipomoea neei | - |
gene hss | - |
Purification (Comment) | Organism |
---|---|
recombinant tagged enzyme from Escherichia coli by affinity chromatography | Ipomoea neei |
recombinant tagged enzyme from Escherichia coli by affinity chromatography | Ipomoea hederifolia |
recombinant tagged enzyme from Escherichia coli by affinity chromatography | Ipomoea alba |
recombinant tagged enzyme from Escherichia coli by affinity chromatography | Ipomoea meyeri |
recombinant tagged enzyme from Escherichia coli by affinity chromatography | Distimake quinquefolius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tissue-specific expression of enzyme HSS | Ipomoea neei | - |
additional information | tissue-specific expression of enzyme HSS | Ipomoea hederifolia | - |
additional information | tissue-specific expression of enzyme HSS | Ipomoea alba | - |
additional information | tissue-specific expression of enzyme HSS | Ipomoea meyeri | - |
additional information | tissue-specific expression of enzyme HSS | Distimake quinquefolius | - |
root | - |
Ipomoea neei | - |
root | - |
Ipomoea hederifolia | - |
root | - |
Ipomoea alba | - |
root | - |
Ipomoea meyeri | - |
root | - |
Distimake quinquefolius | - |
shoot | young | Ipomoea neei | - |
shoot | young | Ipomoea hederifolia | - |
shoot | young | Ipomoea alba | - |
shoot | young | Ipomoea meyeri | - |
shoot | young | Distimake quinquefolius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Ipomoea alba |
0.062 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Ipomoea neei |
0.136 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Ipomoea hederifolia |
0.138 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Ipomoea meyeri |
0.138 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Distimake quinquefolius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + spermidine | - |
Ipomoea neei | sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | - |
Ipomoea hederifolia | sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | - |
Ipomoea alba | sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | - |
Ipomoea meyeri | sym-homospermidine + propane-1,3-diamine | - |
? | |
putrescine + spermidine | - |
Distimake quinquefolius | sym-homospermidine + propane-1,3-diamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41600, about, sequence calculation | Ipomoea neei |
? | x * 41600, about, sequence calculation | Ipomoea hederifolia |
? | x * 41300, about, sequence calculation | Distimake quinquefolius |
? | x * 41500, about, sequence calculation | Ipomoea alba |
? | x * 41500, about, sequence calculation | Ipomoea meyeri |
Synonyms | Comment | Organism |
---|---|---|
HSS | - |
Ipomoea neei |
HSS | - |
Ipomoea hederifolia |
HSS | - |
Ipomoea alba |
HSS | - |
Ipomoea meyeri |
HSS | - |
Distimake quinquefolius |
General Information | Comment | Organism |
---|---|---|
evolution | the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection | Ipomoea neei |
evolution | the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection | Ipomoea hederifolia |
evolution | the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection | Ipomoea alba |
evolution | the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection | Ipomoea meyeri |
evolution | the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection | Distimake quinquefolius |
metabolism | homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis | Ipomoea neei |
metabolism | homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis | Ipomoea hederifolia |
metabolism | homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis | Ipomoea alba |
metabolism | homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis | Ipomoea meyeri |
metabolism | homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis | Distimake quinquefolius |
additional information | the Convulvulaceae species Ipomoea alba contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality | Ipomoea alba |
additional information | the Convulvulaceae species Ipomoea hederifolia contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality | Ipomoea hederifolia |
additional information | the Convulvulaceae species Ipomoea meyeri contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality | Ipomoea meyeri |
additional information | the Convulvulaceae species Ipomoea neei contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality | Ipomoea neei |
additional information | the Convulvulaceae species Merremia quinquefolia contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality | Distimake quinquefolius |