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Literature summary for 2.5.1.45 extracted from

  • Kaltenegger, E.; Eich, E.; Ober, D.
    Evolution of homospermidine synthase in the convolvulaceae: a story of gene duplication, gene loss, and periods of various selection pressures (2013), Plant Cell, 25, 1213-1227.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea neei
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea hederifolia
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea alba
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea meyeri
gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Distimake quinquefolius

Protein Variants

Protein Variants Comment Organism
I277D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and slightly reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
I277D/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
N266H site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
N266H/I277D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine, but unaltered deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
N266H/I277D/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
N266H/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
N281D site-directed mutagenesis, the mutant shows slightly reduced HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41300
-
-
Distimake quinquefolius
41500
-
-
Ipomoea alba
41500
-
-
Ipomoea meyeri
41600
-
x * 41600, about, sequence calculation Ipomoea neei
41600
-
x * 41600, about, sequence calculation Ipomoea hederifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
putrescine + spermidine Ipomoea neei
-
sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine Ipomoea hederifolia
-
sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine Ipomoea alba
-
sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine Ipomoea meyeri
-
sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine Distimake quinquefolius
-
sym-homospermidine + propane-1,3-diamine
-
?

Organism

Organism UniProt Comment Textmining
Distimake quinquefolius
-
gene hss
-
Ipomoea alba
-
gene hss
-
Ipomoea hederifolia
-
gene hss
-
Ipomoea meyeri
-
gene hss
-
Ipomoea neei
-
gene hss
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea neei
recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea hederifolia
recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea alba
recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea meyeri
recombinant tagged enzyme from Escherichia coli by affinity chromatography Distimake quinquefolius

Source Tissue

Source Tissue Comment Organism Textmining
additional information tissue-specific expression of enzyme HSS Ipomoea neei
-
additional information tissue-specific expression of enzyme HSS Ipomoea hederifolia
-
additional information tissue-specific expression of enzyme HSS Ipomoea alba
-
additional information tissue-specific expression of enzyme HSS Ipomoea meyeri
-
additional information tissue-specific expression of enzyme HSS Distimake quinquefolius
-
root
-
Ipomoea neei
-
root
-
Ipomoea hederifolia
-
root
-
Ipomoea alba
-
root
-
Ipomoea meyeri
-
root
-
Distimake quinquefolius
-
shoot young Ipomoea neei
-
shoot young Ipomoea hederifolia
-
shoot young Ipomoea alba
-
shoot young Ipomoea meyeri
-
shoot young Distimake quinquefolius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea alba
0.062
-
purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea neei
0.136
-
purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea hederifolia
0.138
-
purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea meyeri
0.138
-
purified recombinant enzyme, pH and temperature not specified in the publication Distimake quinquefolius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
putrescine + spermidine
-
Ipomoea neei sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine
-
Ipomoea hederifolia sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine
-
Ipomoea alba sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine
-
Ipomoea meyeri sym-homospermidine + propane-1,3-diamine
-
?
putrescine + spermidine
-
Distimake quinquefolius sym-homospermidine + propane-1,3-diamine
-
?

Subunits

Subunits Comment Organism
? x * 41600, about, sequence calculation Ipomoea neei
? x * 41600, about, sequence calculation Ipomoea hederifolia
? x * 41300, about, sequence calculation Distimake quinquefolius
? x * 41500, about, sequence calculation Ipomoea alba
? x * 41500, about, sequence calculation Ipomoea meyeri

Synonyms

Synonyms Comment Organism
HSS
-
Ipomoea neei
HSS
-
Ipomoea hederifolia
HSS
-
Ipomoea alba
HSS
-
Ipomoea meyeri
HSS
-
Distimake quinquefolius

General Information

General Information Comment Organism
evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea neei
evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea hederifolia
evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea alba
evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea meyeri
evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Distimake quinquefolius
metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea neei
metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea hederifolia
metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea alba
metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea meyeri
metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Distimake quinquefolius
additional information the Convulvulaceae species Ipomoea alba contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea alba
additional information the Convulvulaceae species Ipomoea hederifolia contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea hederifolia
additional information the Convulvulaceae species Ipomoea meyeri contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea meyeri
additional information the Convulvulaceae species Ipomoea neei contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea neei
additional information the Convulvulaceae species Merremia quinquefolia contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality Distimake quinquefolius