BRENDA - Enzyme Database show
show all sequences of 2.5.1.44

Homospermidine synthase of Rhodopseudomonas viridis: substrate specificity and effects of the heterologously expressed enzyme of polyamine metabolism of Escherichia coli

Ober, D.; Tholl, D.; Martin, W.; Hartmann, T.; J. Gen. Appl. Microbiol. 42, 411-420 (1996)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
NAD+
-
Blastochloris viridis
0.27
-
putrescine
-
Blastochloris viridis
1.5
-
spermidine
in presence of 1 mM putrescine
Blastochloris viridis
1.7
-
putrescine
-
Blastochloris viridis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
activates, optimal concentration at 50 mM
Blastochloris viridis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Blastochloris viridis
-
sym-homospermidine + NH3
-
Blastochloris viridis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Blastochloris viridis
-
accession no. L77975; DSM 134; expressed in Escherichia coli strain BL21/pHsRvT72.2
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture medium
-
Blastochloris viridis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
-
288702
Blastochloris viridis
sym-homospermidine + NH3
-
288702
Blastochloris viridis
?
putrescine + 1,3-diaminopropane
-
288702
Blastochloris viridis
homospermidine + spermidine
-
288702
Blastochloris viridis
-
putrescine + 1,6 diaminohexane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
288702
Blastochloris viridis
?
putrescine + 1,7-diaminoheptane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane
-
288702
Blastochloris viridis
-
putrescine + cadaverine
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
288702
Blastochloris viridis
?
spermidine
-
288702
Blastochloris viridis
homospermidine + putrescine + 1,3-diaminopropane
-
288702
Blastochloris viridis
?
spermidine + 1,6-diaminohexane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
288702
Blastochloris viridis
?
spermidine + cadaverine
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
288702
Blastochloris viridis
?
spermidine + putrescine
-
288702
Blastochloris viridis
homospermidine + 1,3-diaminopropane
-
288702
Blastochloris viridis
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
increasing activity up to 45°C, sharp decrease at higher temperatures
Blastochloris viridis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-18
-
storage as acetone dry-powder, stable for several months
Blastochloris viridis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
9
in 50 mM potassium phosphate or Bis-Tris-propane buffers
Blastochloris viridis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Blastochloris viridis
NADP+
68% of the activity with NAD+
Blastochloris viridis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Blastochloris viridis
NADP+
68% of the activity with NAD+
Blastochloris viridis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
NAD+
-
Blastochloris viridis
0.27
-
putrescine
-
Blastochloris viridis
1.5
-
spermidine
in presence of 1 mM putrescine
Blastochloris viridis
1.7
-
putrescine
-
Blastochloris viridis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
activates, optimal concentration at 50 mM
Blastochloris viridis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Blastochloris viridis
-
sym-homospermidine + NH3
-
Blastochloris viridis
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture medium
-
Blastochloris viridis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
-
288702
Blastochloris viridis
sym-homospermidine + NH3
-
288702
Blastochloris viridis
?
putrescine + 1,3-diaminopropane
-
288702
Blastochloris viridis
homospermidine + spermidine
-
288702
Blastochloris viridis
-
putrescine + 1,6 diaminohexane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
288702
Blastochloris viridis
?
putrescine + 1,7-diaminoheptane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane
-
288702
Blastochloris viridis
-
putrescine + cadaverine
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
288702
Blastochloris viridis
?
spermidine
-
288702
Blastochloris viridis
homospermidine + putrescine + 1,3-diaminopropane
-
288702
Blastochloris viridis
?
spermidine + 1,6-diaminohexane
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
288702
Blastochloris viridis
?
spermidine + cadaverine
-
288702
Blastochloris viridis
homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
288702
Blastochloris viridis
?
spermidine + putrescine
-
288702
Blastochloris viridis
homospermidine + 1,3-diaminopropane
-
288702
Blastochloris viridis
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
increasing activity up to 45°C, sharp decrease at higher temperatures
Blastochloris viridis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-18
-
storage as acetone dry-powder, stable for several months
Blastochloris viridis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
9
in 50 mM potassium phosphate or Bis-Tris-propane buffers
Blastochloris viridis
Other publictions for EC 2.5.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739378
Lopez-Gomez
-
Homospermidine synthase contri ...
Rhizobium tropici, Rhizobium tropici Rt CIAT899
Plant Soil
404
413-425
2016
-
-
1
-
1
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739672
Krossa
Comprehensive structural chara ...
Blastochloris viridis
Sci. Rep.
6
19501
2016
-
-
-
1
2
-
-
-
-
1
-
2
-
5
-
-
-
2
-
-
-
-
6
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
3
3
-
-
-
739108
Li
Different polyamine pathways f ...
Paramecium tetraurelia, Paramecium tetraurelia d4-2
Mol. Microbiol.
97
791-807
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
704662
Shaw
Evolution and multifarious hor ...
Acinetobacter tartarogenes, Blastochloris viridis, Bradyrhizobium japonicum, Opitutus terrae, Paramecium tetraurelia, Ralstonia phage phiRSL1, Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
-
-
7
-
-
-
-
3
-
-
-
-
-
15
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
7
-
-
-
-
-
7
7
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
-
-
1
1
-
3
3
288701
Tholl
Purification, molecular clonin ...
Blastochloris viridis
Eur. J. Biochem.
240
373-379
1996
-
-
1
-
-
-
-
1
-
-
3
1
-
6
-
-
1
-
-
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
3
1
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288702
Ober
-
Homospermidine synthase of Rho ...
Blastochloris viridis
J. Gen. Appl. Microbiol.
42
411-420
1996
-
-
-
-
-
-
-
4
-
1
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288699
Böttcher
-
Biosynthesis of pyrrolizidine ...
Blastochloris viridis
Can. J. Chem.
72
80-85
1994
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
1
1
1
9
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
1
-
-
-
1
-
1
1
1
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288700
Yamamoto
Purification and characterizat ...
Acinetobacter tartarogenes
J. Biochem.
114
45-49
1993
-
-
-
-
-
-
3
1
-
1
2
1
-
6
-
-
1
-
-
-
1
1
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
-
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288698
Srivenugopal
Enzymic synthesis of sym-homos ...
Lathyrus sativus, Santalum album
Biochem. J.
190
461-464
1980
-
-
-
-
-
-
4
1
-
-
1
2
-
2
-
-
1
-
-
2
2
-
2
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
1
2
-
-
-
1
-
2
2
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288697
Tait
The formation of homospermidin ...
Blastochloris viridis, Blastochloris viridis N.C.I.B. 10028
Biochem. Soc. Trans.
7
199-200
1979
-
-
-
-
-
-
4
2
-
3
1
2
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
3
1
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-