BRENDA - Enzyme Database show
show all sequences of 2.5.1.44

Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings

Srivenugopal, K.S.; Adiga, P.R.; Biochem. J. 190, 461-464 (1980)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,3-Diaminopropane
-
Lathyrus sativus
cadaverine
-
Lathyrus sativus
N-ethylmaleimide
inhibition suggests, that the enzyme is of a thiol nature
Lathyrus sativus
spermidine
-
Lathyrus sativus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
putrescine
-
Lathyrus sativus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Lathyrus sativus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Lathyrus sativus
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
sym-homospermidine + NH3
-
Lathyrus sativus
?
2 putrescine
Santalum album
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
sym-homospermidine + NH3
-
Santalum album
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lathyrus sativus
-
grass pea
-
Santalum album
-
-
-
Purification (Commentary)
Commentary
Organism
partial, 100fold
Lathyrus sativus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Santalum album
-
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00025
-
-
Lathyrus sativus
0.0038
-
-
Santalum album
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
288698
Lathyrus sativus
sym-homospermidine + NH3
-
288698
Lathyrus sativus
?
2 putrescine
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
288698
Santalum album
sym-homospermidine + NH3
-
288698
Santalum album
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
reaction linear up to 60 min and is proportional to the amount of enzyme protein (0.1-0.5 mg)
Lathyrus sativus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
stable for at least 2 weeks in the presence of 1 mM NAD+
Lathyrus sativus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
in Tris/HCl buffer
Lathyrus sativus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
absolute requirement
Lathyrus sativus
NAD+
-
Santalum album
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
absolute requirement
Lathyrus sativus
NAD+
-
Santalum album
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,3-Diaminopropane
-
Lathyrus sativus
cadaverine
-
Lathyrus sativus
N-ethylmaleimide
inhibition suggests, that the enzyme is of a thiol nature
Lathyrus sativus
spermidine
-
Lathyrus sativus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3
-
putrescine
-
Lathyrus sativus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Lathyrus sativus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Lathyrus sativus
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
sym-homospermidine + NH3
-
Lathyrus sativus
?
2 putrescine
Santalum album
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
sym-homospermidine + NH3
-
Santalum album
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 100fold
Lathyrus sativus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Santalum album
-
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00025
-
-
Lathyrus sativus
0.0038
-
-
Santalum album
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
288698
Lathyrus sativus
sym-homospermidine + NH3
-
288698
Lathyrus sativus
?
2 putrescine
as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45
288698
Santalum album
sym-homospermidine + NH3
-
288698
Santalum album
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
reaction linear up to 60 min and is proportional to the amount of enzyme protein (0.1-0.5 mg)
Lathyrus sativus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
stable for at least 2 weeks in the presence of 1 mM NAD+
Lathyrus sativus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
in Tris/HCl buffer
Lathyrus sativus
Other publictions for EC 2.5.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739378
Lopez-Gomez
-
Homospermidine synthase contri ...
Rhizobium tropici, Rhizobium tropici Rt CIAT899
Plant Soil
404
413-425
2016
-
-
1
-
1
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739672
Krossa
Comprehensive structural chara ...
Blastochloris viridis
Sci. Rep.
6
19501
2016
-
-
-
1
2
-
-
-
-
1
-
2
-
5
-
-
-
2
-
-
-
-
6
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
3
3
-
-
-
739108
Li
Different polyamine pathways f ...
Paramecium tetraurelia, Paramecium tetraurelia d4-2
Mol. Microbiol.
97
791-807
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
704662
Shaw
Evolution and multifarious hor ...
Acinetobacter tartarogenes, Blastochloris viridis, Bradyrhizobium japonicum, Opitutus terrae, Paramecium tetraurelia, Ralstonia phage phiRSL1, Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
-
-
7
-
-
-
-
3
-
-
-
-
-
15
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
7
-
-
-
-
-
7
7
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
-
-
1
1
-
3
3
288701
Tholl
Purification, molecular clonin ...
Blastochloris viridis
Eur. J. Biochem.
240
373-379
1996
-
-
1
-
-
-
-
1
-
-
3
1
-
6
-
-
1
-
-
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
3
1
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288702
Ober
-
Homospermidine synthase of Rho ...
Blastochloris viridis
J. Gen. Appl. Microbiol.
42
411-420
1996
-
-
-
-
-
-
-
4
-
1
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288699
Böttcher
-
Biosynthesis of pyrrolizidine ...
Blastochloris viridis
Can. J. Chem.
72
80-85
1994
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
1
1
1
9
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
1
-
-
-
1
-
1
1
1
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288700
Yamamoto
Purification and characterizat ...
Acinetobacter tartarogenes
J. Biochem.
114
45-49
1993
-
-
-
-
-
-
3
1
-
1
2
1
-
6
-
-
1
-
-
-
1
1
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
-
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288698
Srivenugopal
Enzymic synthesis of sym-homos ...
Lathyrus sativus, Santalum album
Biochem. J.
190
461-464
1980
-
-
-
-
-
-
4
1
-
-
1
2
-
2
-
-
1
-
-
2
2
-
2
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
1
2
-
-
-
1
-
2
2
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288697
Tait
The formation of homospermidin ...
Blastochloris viridis, Blastochloris viridis N.C.I.B. 10028
Biochem. Soc. Trans.
7
199-200
1979
-
-
-
-
-
-
4
2
-
3
1
2
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
3
1
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-