Cloned (Comment) | Organism |
---|---|
gene MxNAS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR expression analysis, recombinant expression in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV 35S promoter, recombinant isozyme MxNAS2 promotes the synthesis of NAS and increases nicotinamine and chlorophyll contents in the tobacco cells. Overexpression of MxNAS2 improves the tolerance to Fe stress in transgenic tobacco, but leads to delayed flowering. Higher levels of MxNAS2 expression in transgenic tobacco contribute to misshapen flowers and increased levels of Fe, Mn, Cu and Zn in leaf and flower. A MxNAS2-GFP fusion protein is targeted into vesicles and cytoplasmic membrane | Malus baccata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | isozyme MxNAS2 is preferentially localized in vesicles and cytoplasmic membrane | Malus baccata | 16020 | - |
vesicle | isozyme MxNAS2 is preferentially localized in vesicles and cytoplasmic membrane | Malus baccata | 31982 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
x * 36200, about, sequence calculation | Malus baccata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | Malus baccata | - |
3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus baccata | A3DUI9 | var. xiaojinensis, gene MxNAS2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Malus baccata | - |
phloem | - |
Malus baccata | - |
root | - |
Malus baccata | - |
seedling | - |
Malus baccata | - |
xylem | low enzyme levels | Malus baccata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | - |
Malus baccata | 3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36200, about, sequence calculation | Malus baccata |
Synonyms | Comment | Organism |
---|---|---|
MxNAS2 | - |
Malus baccata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Malus baccata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Malus baccata | sequence calculation | - |
5.18 |
Organism | Comment | Expression |
---|---|---|
Malus baccata | the expression of MxNAS2 is highly affected by Fe stress and indoleacetic acid treatment, whereas, weakly affected by abscisic acid treatment in seedlings | down |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in nicotinamine biosynthesis. In addition to its role in metal transport in plants, nicotinamine may be involved in the regulation of metal transfer within cells. These results suggest that nicotinamine excess influences the functions of metal-requiring proteins, including some of the transcription factors | Malus baccata |