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Literature summary for 2.5.1.43 extracted from

  • Dreyfus, C.; Lemaire, D.; Mari, S.; Pignol, D.; Arnoux, P.
    Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea (2009), Proc. Natl. Acad. Sci. USA, 106, 16180-16184.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as a C-terminal His tag protein in Escherichia coli BL21 (DE3) Methanothermobacter thermautotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
at 20°C using the hanging drop vapor diffusion method Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
E81Q mutant binds the substrate but not the product Methanothermobacter thermautotrophicus
E81Q/Y107F inactive mutant Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Methanothermobacter thermautotrophicus
-
5'-S-methyl-5'-thioadenosine + nicotianamine
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
Methanothermobacter thermautotrophicus 5'-S-methyl-5'-thioadenosine + nicotianamine
-
?

Synonyms

Synonyms Comment Organism
MtNAS
-
Methanothermobacter thermautotrophicus
NAS
-
Methanothermobacter thermautotrophicus
nicotianamine synthase
-
Methanothermobacter thermautotrophicus