Cloned (Comment) | Organism |
---|---|
overexpressed as a C-terminal His tag protein in Escherichia coli BL21 (DE3) | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
at 20°C using the hanging drop vapor diffusion method | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
E81Q | mutant binds the substrate but not the product | Methanothermobacter thermautotrophicus |
E81Q/Y107F | inactive mutant | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | Methanothermobacter thermautotrophicus | - |
5'-S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Methanothermobacter thermautotrophicus | 5'-S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtNAS | - |
Methanothermobacter thermautotrophicus |
NAS | - |
Methanothermobacter thermautotrophicus |
nicotianamine synthase | - |
Methanothermobacter thermautotrophicus |