Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine
Wilson, B.A.; Bantia, S.; Salituro, G.M.; Reeve, A.M.; Townsend, C.A.; J. Am. Chem. Soc. 110, 8238-8239 (1988)
No PubMed abstract available
Data extracted from this reference:
General Stability
General Stability
Organism
partially purified enzyme is stable after dialysis against 50 mM phosphate buffer, pH 7.5, containing 20% glycerol and 10 mM 2-mercaptoethanol
Nocardia uniformis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + nocardicin E
Nocardia uniformis
enzyme is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nocardia uniformis
-
ssp. tsuyamanensis, ATCC 21806
-
Purification (Commentary)
Purification (Commentary)
Organism
ssp. tsuyamanensis, ATCC 21806, partial
Nocardia uniformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + nocardicin E
-
2083
Nocardia uniformis
5'-methylthioadenosine + isonocardicin A
-
-
-
?
S-adenosyl-L-methionine + nocardicin E
enzyme is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A
2083
Nocardia uniformis
?
-
-
-
?
General Stability (protein specific)
General Stability
Organism
partially purified enzyme is stable after dialysis against 50 mM phosphate buffer, pH 7.5, containing 20% glycerol and 10 mM 2-mercaptoethanol
Nocardia uniformis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + nocardicin E
Nocardia uniformis
enzyme is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ssp. tsuyamanensis, ATCC 21806, partial
Nocardia uniformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + nocardicin E
-
2083
Nocardia uniformis
5'-methylthioadenosine + isonocardicin A
-
-
-
?
S-adenosyl-L-methionine + nocardicin E
enzyme is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A
2083
Nocardia uniformis
?
-
-
-
?
Other publictions for EC 2.5.1.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
2083
Wilson
-
Cell-free biosynthesis of noca ...
Nocardia uniformis
J. Am. Chem. Soc.
110
8238-8239
1988
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