Cloned (Comment) | Organism |
---|---|
expressed using recombinant yeast microsomes | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
(6aS,11aS)-3,6a,9-trihydroxypterocarpan | 30°C | Glycine max | |
0.15 | - |
dimethylallyl diphosphate | 30°C | Glycine max | |
45 | - |
(6aS,11aS)-3,6a,9-trihydroxypterocarpan | using microsomes of cultured soybean cells | Glycine max | |
68 | - |
(6aS,11aS)-3,6a,9-trihydroxypterocarpan | using recombinant yeast microsomes | Glycine max | |
150 | - |
dimethylallyl diphosphate | using recombinant yeast microsomes | Glycine max | |
180 | - |
dimethylallyl diphosphate | using microsomes of cultured soybean cells | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | sequence contains a plastid targeting signal | Glycine max | 9536 | - |
plastid | protein sequence is led by a plastid targeting signal sequence | Glycine max | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 50% activity | Glycine max | |
Mg2+ | 100% activity | Glycine max | |
Mg2+ | requirement for divalent cations, with Mg2+ being more effective than Mn2+ | Glycine max | |
Mn2+ | 68% activity | Glycine max | |
Mn2+ | requirement for divalent cations, with Mg2+ being more effective than Mn2+ | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | B9A1Q4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a plastid targeting signal | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan | - |
Glycine max | 2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan | - |
? | |
dimethylallyl diphosphate + (-)-maackiain | poor substrate | Glycine max | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan | - |
Glycine max | diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan | - |
? | |
additional information | no substrate: genistein and daidzein | Glycine max | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(6aS,11aS)-3,9,6a-trihydroxypterocarpan [(2)-glycinol] 4-dimethylallyltransferase | - |
Glycine max |
G4DT | - |
Glycine max |
pterocarpan 4-dimethylallyltransferase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay | Glycine max |
Organism | Comment | Expression |
---|---|---|
Glycine max | expression is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cell culture | up |
Glycine max | expression of the G4DT gene is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cultured soybean cells | up |