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Literature summary for 2.5.1.35 extracted from

  • Sagami, I.; Ojima, N.; Ogura, K.; Seto, S.
    Aspulvinone dimethylallyltransferase (1985), Methods Enzymol., 110, 320-326.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.025%, activates 1.5times Aspergillus terreus
Tween 80 0.025%, activates 2.3times Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
Aspergillus terreus
Bromophenol blue
-
Aspergillus terreus
diphosphate
-
Aspergillus terreus
lecithin 0.025%, 69% inhibition Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
aspulvinone G
-
Aspergillus terreus
0.0137
-
aspulvinone E
-
Aspergillus terreus
0.04
-
dimethylallyl diphosphate
-
Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
6 * 45000, SDS-PAGE Aspergillus terreus
240000 270000 gel filtration Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
IAM 2054
-
Aspergillus terreus IAM 2054
-
IAM 2054
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus terreus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus terreus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0294
-
-
Aspergillus terreus

Storage Stability

Storage Stability Organism
-20°C, stable for 1 week Aspergillus terreus
4°C, 50% loss of activity after 3 weeks Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
-
Aspergillus terreus ?
-
?
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
-
Aspergillus terreus IAM 2054 ?
-
?
(E)-butenyl diphosphate + aspulvinone E
-
Aspergillus terreus ?
-
?
(E)-butenyl diphosphate + aspulvinone E
-
Aspergillus terreus IAM 2054 ?
-
?
cycloheptylideneethyl diphosphate + aspulvinone E
-
Aspergillus terreus ?
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
-
Aspergillus terreus ?
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
-
Aspergillus terreus IAM 2054 ?
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
-
Aspergillus terreus ?
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
-
Aspergillus terreus IAM 2054 ?
-
?
dimethylallyl diphosphate + aspulvinone E i.e. dihydroxypulvinone Aspergillus terreus diphosphate + aspulvinone H
-
?
dimethylallyl diphosphate + aspulvinone E i.e. dihydroxypulvinone Aspergillus terreus IAM 2054 diphosphate + aspulvinone H
-
?
dimethylallyl diphosphate + aspulvinone G i.e. trihydroxypulvinone, 71% of the activity with aspulvinone E Aspergillus terreus ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Aspergillus terreus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0178
-
diphosphate
-
Aspergillus terreus
0.05
-
diphosphate
-
Aspergillus terreus