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Literature summary for 2.5.1.35 extracted from
- Purification and characterization of dimethylallyl pyrophosphate: aspulvinone dimethylallyltransferase from Aspergillus terreus (1978), Biochemistry, 17, 2696-2702.
General Stability
| General Stability | Organism |
|---|---|
| addition of 2-mercaptoethanol does not affect stability | Aspergillus terreus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aspulvinone E dimethyl ether | - |
Aspergillus terreus |  |
| Bromophenol blue | - |
Aspergillus terreus | |
| diphosphate | noncompetitive against aspulvinone, mixed type against dimethylallyl diphosphate | Aspergillus terreus | |
| isopentenyl diphosphate | - |
Aspergillus terreus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0077 | - |
aspulvinone G | - |
Aspergillus terreus | |
| 0.0137 | - |
aspulvinone E | - |
Aspergillus terreus | |
| 0.04 | - |
dimethylallyl diphosphate | - |
Aspergillus terreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Aspergillus terreus |
| 240000 | 270000 | gel filtration | Aspergillus terreus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus terreus | enzyme is involved in biosynthesis of aspulvinone pigments | ? | - |
? | |
| additional information | Aspergillus terreus IAM 2054 | enzyme is involved in biosynthesis of aspulvinone pigments | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
IAM 2054 | - |
| Aspergillus terreus IAM 2054 | - |
IAM 2054 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus terreus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus terreus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0294 | - |
- |
Aspergillus terreus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 week | Aspergillus terreus |
| 4°C, 50% loss of activity after 3 weeks | Aspergillus terreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-3-methylpent-2-enyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
| (E)-3-methylpent-2-enyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
| (E)-butenyl diphosphate + aspulvinone E | 10% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
| cycloheptylideneethyl diphosphate + aspulvinone E | 10% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
| cyclohexylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
| cyclohexylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
| cyclopentylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
| cyclopentylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
| dimethylallyl diphosphate + aspulvinone E | - |
Aspergillus terreus | diphosphate + aspulvinone H | - |
? | |
| dimethylallyl diphosphate + aspulvinone E | - |
Aspergillus terreus IAM 2054 | diphosphate + aspulvinone H | - |
? | |
| dimethylallyl diphosphate + aspulvinone G | - |
Aspergillus terreus | ? | - |
? | |
| dimethylallyl diphosphate + aspulvinone I | 75% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
| dimethylallyl diphosphate + aspulvinone I-c | 13% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
| dimethylallyl diphosphate + atromentic acid | 7% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
| dimethylallyl diphosphate + pulvinone | 6% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
| additional information | enzyme is involved in biosynthesis of aspulvinone pigments | Aspergillus terreus | ? | - |
? | |
| additional information | enzyme is involved in biosynthesis of aspulvinone pigments | Aspergillus terreus IAM 2054 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Aspergillus terreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus terreus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0178 | - |
diphosphate | inhibition against dimethylallyl diphosphate | Aspergillus terreus | |
| 0.05 | - |
diphosphate | inhibition against aspulvinone | Aspergillus terreus | |
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