BRENDA - Enzyme Database
show all sequences of 2.5.1.35

Purification and characterization of dimethylallyl pyrophosphate: aspulvinone dimethylallyltransferase from Aspergillus terreus

Takahashi, I.; Ojima, N.; Ogura, K.; Seto, S.; Biochemistry 17, 2696-2702 (1978)

Data extracted from this reference:

General Stability
General Stability
Organism
addition of 2-mercaptoethanol does not affect stability
Aspergillus terreus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Aspulvinone E dimethyl ether
-
Aspergillus terreus
Bromophenol blue
-
Aspergillus terreus
diphosphate
noncompetitive against aspulvinone, mixed type against dimethylallyl diphosphate
Aspergillus terreus
isopentenyl diphosphate
-
Aspergillus terreus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
aspulvinone G
-
Aspergillus terreus
0.0137
-
aspulvinone E
-
Aspergillus terreus
0.04
-
dimethylallyl diphosphate
-
Aspergillus terreus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
6 * 45000, SDS-PAGE
Aspergillus terreus
240000
270000
gel filtration
Aspergillus terreus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Aspergillus terreus
enzyme is involved in biosynthesis of aspulvinone pigments
?
-
-
?
additional information
Aspergillus terreus IAM 2054
enzyme is involved in biosynthesis of aspulvinone pigments
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus terreus
-
IAM 2054
-
Aspergillus terreus IAM 2054
-
IAM 2054
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Aspergillus terreus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus terreus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0294
-
-
Aspergillus terreus
Storage Stability
Storage Stability
Organism
-20°C, stable for 1 week
Aspergillus terreus
4°C, 50% loss of activity after 3 weeks
Aspergillus terreus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
(E)-butenyl diphosphate + aspulvinone E
10% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cycloheptylideneethyl diphosphate + aspulvinone E
10% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone E
-
636862
Aspergillus terreus
diphosphate + aspulvinone H
-
636862
Aspergillus terreus
?
dimethylallyl diphosphate + aspulvinone E
-
636862
Aspergillus terreus IAM 2054
diphosphate + aspulvinone H
-
636862
Aspergillus terreus IAM 2054
?
dimethylallyl diphosphate + aspulvinone G
-
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone I
75% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone I-c
13% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + atromentic acid
7% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + pulvinone
6% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
additional information
enzyme is involved in biosynthesis of aspulvinone pigments
636862
Aspergillus terreus
?
-
-
-
?
additional information
enzyme is involved in biosynthesis of aspulvinone pigments
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
hexamer
6 * 45000, SDS-PAGE
Aspergillus terreus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Aspergillus terreus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0178
-
diphosphate
inhibition against dimethylallyl diphosphate
Aspergillus terreus
0.05
-
diphosphate
inhibition against aspulvinone
Aspergillus terreus
General Stability (protein specific)
General Stability
Organism
addition of 2-mercaptoethanol does not affect stability
Aspergillus terreus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Aspulvinone E dimethyl ether
-
Aspergillus terreus
Bromophenol blue
-
Aspergillus terreus
diphosphate
noncompetitive against aspulvinone, mixed type against dimethylallyl diphosphate
Aspergillus terreus
isopentenyl diphosphate
-
Aspergillus terreus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0178
-
diphosphate
inhibition against dimethylallyl diphosphate
Aspergillus terreus
0.05
-
diphosphate
inhibition against aspulvinone
Aspergillus terreus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
aspulvinone G
-
Aspergillus terreus
0.0137
-
aspulvinone E
-
Aspergillus terreus
0.04
-
dimethylallyl diphosphate
-
Aspergillus terreus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
6 * 45000, SDS-PAGE
Aspergillus terreus
240000
270000
gel filtration
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Aspergillus terreus
enzyme is involved in biosynthesis of aspulvinone pigments
?
-
-
?
additional information
Aspergillus terreus IAM 2054
enzyme is involved in biosynthesis of aspulvinone pigments
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus terreus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus terreus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0294
-
-
Aspergillus terreus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for 1 week
Aspergillus terreus
4°C, 50% loss of activity after 3 weeks
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
(E)-butenyl diphosphate + aspulvinone E
10% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cycloheptylideneethyl diphosphate + aspulvinone E
10% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclohexylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus
?
-
-
-
?
cyclopentylideneethyl diphosphate + aspulvinone E
35% of the activity with dimethylallyl diphosphate
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone E
-
636862
Aspergillus terreus
diphosphate + aspulvinone H
-
636862
Aspergillus terreus
?
dimethylallyl diphosphate + aspulvinone E
-
636862
Aspergillus terreus IAM 2054
diphosphate + aspulvinone H
-
636862
Aspergillus terreus IAM 2054
?
dimethylallyl diphosphate + aspulvinone G
-
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone I
75% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + aspulvinone I-c
13% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + atromentic acid
7% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
dimethylallyl diphosphate + pulvinone
6% of the activity with aspulvinone E
636862
Aspergillus terreus
?
-
-
-
?
additional information
enzyme is involved in biosynthesis of aspulvinone pigments
636862
Aspergillus terreus
?
-
-
-
?
additional information
enzyme is involved in biosynthesis of aspulvinone pigments
636862
Aspergillus terreus IAM 2054
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 45000, SDS-PAGE
Aspergillus terreus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Aspergillus terreus
Other publictions for EC 2.5.1.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636863
Sagami
Aspulvinone dimethylallyltrans ...
Aspergillus terreus, Aspergillus terreus IAM 2054
Methods Enzymol.
110
320-326
1985
2
-
-
-
-
-
4
3
-
-
2
-
-
3
-
-
1
-
-
1
1
2
12
1
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
4
2
3
-
-
2
-
-
-
-
1
-
1
1
2
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636862
Takahashi
Purification and characterizat ...
Aspergillus terreus, Aspergillus terreus IAM 2054
Biochemistry
17
2696-2702
1978
-
-
-
-
-
1
4
3
-
-
2
2
-
6
-
-
1
-
-
2
1
2
17
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
4
2
3
-
-
2
2
-
-
-
1
-
2
1
2
17
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-