Application | Comment | Organism |
---|---|---|
biotechnology | the combination of the two dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) can be successfully used for chemoenzymatic synthesis of the diprenylated derivatives. The potential of recombinant enzymes from secondary metabolite biosynthesis as promising tools for the production of designed compounds is demonstrated | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
expressed as a recombinant protein | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
two dimethylallyltryptophan synthases, FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80), which catalyse the prenylation of L-tryptophan at positions C4 and C7, respectively, are identified in Aspergillus fumigatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 11-methyl-DL-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 33.5% (FgaPT2 followed by 7-DMATS), 4.8% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 10.3% (FgaPT2 followed by 7-DMATS), 3.5% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 24.3% (FgaPT2 followed by 7-DMATS), 0.9% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + 6-methyl-DL-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 2.6% (FgaPT2 followed by 7-DMATS), 1.5% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + D-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 4.3% (FgaPT2 followed by 7-DMATS), 2.6% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + DL-indole-3-lactic acid | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 35.7% (FgaPT2 followed by 7-DMATS), 16.7% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-abrine | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 92.7% (FgaPT2 followed by 7-DMATS), 11.3% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-beta-homotryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 31.5% (FgaPT2 followed by 7-DMATS), 6.6% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-tryptophan | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 89.7% (FgaPT2 followed by 7-DMATS), 20% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + tryptamine | tandem incubation of dimethylallyltryptophan synthases FgaPT2 and 7-DMATS (EC 2.5.1.34 and EC 2.5.1.80) leads to the production of diprenylated indole derivatives. Yield of diprenylated product: 11% (FgaPT2 followed by 7-DMATS), 6.3% (7-DMATS followed by FgaPT2) | Aspergillus fumigatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
FgaPT2 | - |
Aspergillus fumigatus |