Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Malbranchea aurantiaca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 50-80% inhibition | Malbranchea aurantiaca | |
D-tryptophan | competitive with L-tryptophan | Malbranchea aurantiaca | |
EDTA | 5 mM, inactivation. Regeneration of activity by dialysis with reaction buffer ontaining MgCl2 | Malbranchea aurantiaca | |
Fe2+ | 5 mM, 50-80% inhibition | Malbranchea aurantiaca | |
Mn2+ | 5 mM, 50-80% inhibition | Malbranchea aurantiaca | |
Ni2+ | 5 mM, 50-80% inhibition | Malbranchea aurantiaca | |
Zn2+ | 5 mM, 50-80% inhibition | Malbranchea aurantiaca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04888 | - |
L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca | |
0.106 | - |
Nalpha-methyl-L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca | |
0.1078 | - |
L-tryptophan | presence of D-tryptophan, pH 7.4, 30°C | Malbranchea aurantiaca | |
0.3031 | - |
5-hydroxy-L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation to 130% of initial activity | Malbranchea aurantiaca | |
Mg2+ | stimulation to 130% of initial activity | Malbranchea aurantiaca | |
additional information | activity does not depend on addition of divalent cation cofactor | Malbranchea aurantiaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein | Malbranchea aurantiaca |
52000 | - |
1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein | Malbranchea aurantiaca |
61600 | - |
gel filtration | Malbranchea aurantiaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malbranchea aurantiaca | B1A4C4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 1-methyl-L-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan | 3.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan | 17.0% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-methyl-D-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-D-tryptophan | 0.5% of the activity with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 16.1% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-methyl-L-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 27.0% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + L-tryptophan | - |
Malbranchea aurantiaca | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan | i.e. L-abrine | Malbranchea aurantiaca | diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan | 24.9% of the yield with L-tryptophan | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein | Malbranchea aurantiaca |
Synonyms | Comment | Organism |
---|---|---|
MaPT | - |
Malbranchea aurantiaca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Malbranchea aurantiaca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
5-hydroxy-L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca | |
1.06 | - |
Nalpha-methyl-L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca | |
1.34 | - |
L-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca | |
1.4 | - |
L-tryptophan | presence of D-tryptophan, pH 7.4, 30°C | Malbranchea aurantiaca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Malbranchea aurantiaca |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0404 | - |
D-tryptophan | pH 7.4, 30°C | Malbranchea aurantiaca |