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Literature summary for 2.5.1.34 extracted from

  • Ding, Y.; Williams, R.M.; Sherman, D.H.
    Molecular analysis of a 4-dimethylallyltryptophan synthase from Malbranchea aurantiaca (2008), J. Biol. Chem., 283, 16068-16076.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Malbranchea aurantiaca

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
D-tryptophan competitive with L-tryptophan Malbranchea aurantiaca
EDTA 5 mM, inactivation. Regeneration of activity by dialysis with reaction buffer ontaining MgCl2 Malbranchea aurantiaca
Fe2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
Mn2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
Ni2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
Zn2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04888
-
L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
0.106
-
Nalpha-methyl-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
0.1078
-
L-tryptophan presence of D-tryptophan, pH 7.4, 30°C Malbranchea aurantiaca
0.3031
-
5-hydroxy-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation to 130% of initial activity Malbranchea aurantiaca
Mg2+ stimulation to 130% of initial activity Malbranchea aurantiaca
additional information activity does not depend on addition of divalent cation cofactor Malbranchea aurantiaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca
52000
-
1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca
61600
-
gel filtration Malbranchea aurantiaca

Organism

Organism UniProt Comment Textmining
Malbranchea aurantiaca B1A4C4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
Malbranchea aurantiaca diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan 3.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
Malbranchea aurantiaca diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan 17.0% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methyl-D-tryptophan
-
Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-D-tryptophan 0.5% of the activity with L-tryptophan ?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
Malbranchea aurantiaca diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 16.1% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methyl-L-tryptophan
-
Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan
-
?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
Malbranchea aurantiaca diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 27.0% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-tryptophan
-
Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Malbranchea aurantiaca diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan 24.9% of the yield with L-tryptophan ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca

Synonyms

Synonyms Comment Organism
MaPT
-
Malbranchea aurantiaca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Malbranchea aurantiaca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
5-hydroxy-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
1.06
-
Nalpha-methyl-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
1.34
-
L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
1.4
-
L-tryptophan presence of D-tryptophan, pH 7.4, 30°C Malbranchea aurantiaca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Malbranchea aurantiaca

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0404
-
D-tryptophan pH 7.4, 30°C Malbranchea aurantiaca