Cloned (Comment) | Organism |
---|---|
into pGEM-T and subsequently into pQE60 for expression in Escherichia coli XL1 Blue MRF cells | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
determined by SDS-PAGE | Aspergillus fumigatus |
54000 | - |
calculated for His6-7-DMATS | Aspergillus fumigatus |
65000 | - |
determined by gel filtration | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
isoform FgaPT2 | - |
Aspergillus fumigatus | Q50EL0 | - |
- |
Purification (Comment) | Organism |
---|---|
His6-7-DMATS is purified with Ni-NTA agarose | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 1-methyl-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan | 0.4% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 11-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 11-methyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan | 1.6% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 11-methyl-DL-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + 4-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-DL-tryptophan + ? | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-bromo-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-bromo-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-fluoro-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-fluoro-4-(3-methylbut-2-enyl)-L-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan | 21.0% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methoxy-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.6% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 8.1% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-fluoro-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 15.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + 7-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan + ? | 16.3% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | diphosphate + D-4-(3-methylbut-2-enyl)-tryptophan | 9.7% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid | 10.5% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + indole-3-acetic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-acetic acid | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + indole-3-acetic acid | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + indole-3-butyric acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-butyric acid | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + indole-3-butyric acid | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid | 32.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid | 29.4% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-abrine | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-beta-homotryptophan | 51.5% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + L-4-(3-methylbut-2-enyl)-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan hydroxamate | 22.4% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester | 7.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + N-acetyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan | 0.2% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan | i.e. L-abrine | Aspergillus fumigatus | diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan | 58.0% of the yield with L-tryptophan | ? | |
dimethylallyl diphosphate + tryptamin | - |
Aspergillus fumigatus | ? | - |
? | |
dimethylallyl diphosphate + tryptamine | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-tryptamine | 4.6% of the yield with L-tryptophan | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
7-dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
7-DMATS | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
enzyme assay | Aspergillus fumigatus |