Crystallization (Comment) | Organism |
---|---|
- |
Claviceps sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Claviceps sp. | |
2,2'-bipyridyl | - |
Claviceps sp. | |
8-hydroxyquinoline | - |
Claviceps sp. | |
8-mercaptoquinoline | - |
Claviceps sp. | |
Acetylacetone | - |
Claviceps sp. | |
Chelating agents | in presence of excess of Ca2+ | Claviceps sp. | |
dithiothreitol | - |
Claviceps sp. | |
EDTA | - |
Claviceps sp. | |
nitrilotriacetic acid | - |
Claviceps sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ion, free in solution not required for activity | Claviceps sp. | |
additional information | enzyme contains a divalent cation which is most likely involved in catalysis | Claviceps sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Claviceps sp. |
70000 | - |
gel filtration | Claviceps sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps sp. | - |
SD58 | - |
Claviceps sp. SD58 | - |
SD58 | - |
Purification (Comment) | Organism |
---|---|
- |
Claviceps sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | random sequential mechanism | Claviceps sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
- |
Claviceps sp. |
Storage Stability | Organism |
---|---|
-20°C, 50% loss of activity after several weeks | Claviceps sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. SD58 | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Claviceps sp. |