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Literature summary for 2.5.1.34 extracted from

  • Cress, W.A.; Chayet, L.T.; Rilling, H.C.
    Crystallization and partial characterization of dimethylallyl pyrophosphate: L-tryptophan dimethylallyltransferase from Claviceps sp. SD58 (1981), J. Biol. Chem., 256, 10917-10923.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Claviceps sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Claviceps sp.
2,2'-bipyridyl
-
Claviceps sp.
8-hydroxyquinoline
-
Claviceps sp.
8-mercaptoquinoline
-
Claviceps sp.
Acetylacetone
-
Claviceps sp.
Chelating agents in presence of excess of Ca2+ Claviceps sp.
dithiothreitol
-
Claviceps sp.
EDTA
-
Claviceps sp.
nitrilotriacetic acid
-
Claviceps sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ion, free in solution not required for activity Claviceps sp.
additional information enzyme contains a divalent cation which is most likely involved in catalysis Claviceps sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Claviceps sp.
70000
-
gel filtration Claviceps sp.

Organism

Organism UniProt Comment Textmining
Claviceps sp.
-
SD58
-
Claviceps sp. SD58
-
SD58
-

Purification (Commentary)

Purification (Comment) Organism
-
Claviceps sp.

Reaction

Reaction Comment Organism Reaction ID
dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan random sequential mechanism Claviceps sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
-
Claviceps sp.

Storage Stability

Storage Stability Organism
-20°C, 50% loss of activity after several weeks Claviceps sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
-
Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan
-
Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Claviceps sp.