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Literature summary for 2.5.1.34 extracted from

  • Gebler, J.C.; Poulter, C.D.
    Purification and characterization of dimethylallyl tryptophan synthase from Claviceps purpurea (1992), Arch. Biochem. Biophys., 296, 308-313.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
dimethylallyl diphosphate pH 7.8, 30°C, in presence of 4 mM CaCl2 Claviceps purpurea
0.012
-
L-tryptophan pH 7.8, 30°C, in presence of 4 mM MgCl2 Claviceps purpurea
0.014
-
dimethylallyl diphosphate
-
Claviceps purpurea
0.017
-
L-tryptophan pH 7.8, 30°C, in presence of 4 mM CaCl2 Claviceps purpurea
0.04
-
L-tryptophan pH 7.8, 30°C Claviceps purpurea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal stimulation at 4-10 mM Claviceps purpurea
Mg2+ maximal stimulation at 4-10 mM Claviceps purpurea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
alpha2, 2 * 53000, SDS-PAGE Claviceps purpurea
105000
-
gel filtration Claviceps purpurea

Organism

Organism UniProt Comment Textmining
Claviceps purpurea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Claviceps purpurea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Claviceps purpurea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
-
Claviceps purpurea

Storage Stability

Storage Stability Organism
-20°C, glycerol-containing buffer, stable for more than 6 months Claviceps purpurea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
-
Claviceps purpurea diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 53000, SDS-PAGE Claviceps purpurea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Claviceps purpurea