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Literature summary for 2.5.1.32 extracted from

  • Camagna, M.; Welsch, R.
    Expression, Purification, and enzyme activity assay of phytoene synthase in vitro (2020), Methods Mol. Biol., 2083, 39-52 .
    View publication on PubMed
Search for more literature for 2.5.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 geranylgeranyl diphosphate Arabidopsis thaliana
-
 15-cis-phytoene + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P37271
-
-

Purification (Commentary)

Purification (Comment) Organism
the purification protocol presents detailed instructions how to purify several milligrams of active enzyme from bacterial lysates, which includes initial recombinant enzyme enrichment through inclusion body purification, chaotropic unfolding, refolding in presence of detergents and purification through immobilized metal affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 geranylgeranyl diphosphate
-
 Arabidopsis thaliana 15-cis-phytoene + 2 diphosphate
-
 ?
2 geranylgeranyl diphosphate overall reaction Arabidopsis thaliana 15-cis-phytoene + 2 diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
phytoene synthase
-
 Arabidopsis thaliana
PSY
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function phytoene synthase catalyzes a rate-limiting step in carotenoid biosynthesis Arabidopsis thaliana