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Literature summary for 2.5.1.32 extracted from

  • Kato, S.; Takaichi, S.; Ishikawa, T.; Asahina, M.; Takahashi, S.; Shinomura, T.
    Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoene synthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis (2016), BMC Plant Biol., 16, 4-15.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene crtB, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information no predictable plastid transit peptide in the N-terminal region of EgCrtB. The stigma of the alga is surrounded by a single membrane and has no structural similarity to the chloroplast. This suggests that EgCrtB might be transported to stigmata as well as plastids and that EgCrtB might contain an as-yet unidentified signal sequence Euglena gracilis
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Organism

Organism UniProt Comment Textmining
Euglena gracilis A0A0U5ARE7 gene crtB
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Euglena gracilis Z A0A0U5ARE7 gene crtB
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Synonyms

Synonyms Comment Organism
EgcrtB
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 Euglena gracilis
phytoene synthase
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 Euglena gracilis

Expression

Organism Comment Expression
Euglena gracilis the expression of gene crtB in cells cultured at 25°C under continuous illumination increases 1.3fold relative to control up

General Information

General Information Comment Organism
evolution Euglena gracilis CrtB belongs to a clade that is distinct from groups of algae and higher plants Euglena gracilis
metabolism the enzyme catalyzes an early step of carotenoid biosynthesis. The carotenoid biosynthetic pathway responds to excess light stress at the level of crtB transcription Euglena gracilis