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Literature summary for 2.5.1.31 extracted from

  • Ohnuma, S.; Koyama, T.; Ogura, K.
    Kinetic studies on the prenyl chain elongation by undecaprenyl diphosphate synthase with artificial substrate homologues (1989), FEBS Lett., 257, 71-74.
    View publication on PubMed
Search for more literature for 2.5.1.31

Inhibitors

Inhibitors Comment Organism Structure
(2E,6E,10E)-4-methylfarnesyl diphosphate competitive inhibitor against (2Z,6E,10E)-geranylgeranyl diphosphate Bacillus subtilis
(2Z,6E,10E)-4-methyl-geranylgeranyl diphosphate allylic substrate homologue, competitive inhibitor against the allylic primer. It also acts as a strong noncompetitive inhibitor against isopentenyl diphosphate Bacillus subtilis
(E)-3-methyl-3-pentenyl diphosphate reactive as substrate and competitive inhibition profile Bacillus subtilis
additional information Z-isomer ZHIPP, (Z)-3-methyl-3-pentenyl diphosphate, which is not accepted as substrate by the enzyme, exhibited no inhibition on the synthase reaction Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
 isopentenyl diphosphate 37°C, pH 37°C Bacillus subtilis
0.0089
-
 (2Z,6E,10E)-geranylgeranyl diphosphate 37°C, pH 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis kinetic experiments with artificial substrate homologues ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Bacillus subtilis 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
(E)-3-methyl-3-pentenyl diphosphate + isopentenyl diphosphate reactive as substrate and competitive inhibition profile Bacillus subtilis ?
-
 ?
additional information kinetic experiments with artificial substrate homologues Bacillus subtilis ?
-
 ?
additional information Z-isomer ZHIPP, (Z)-3-methyl-3-pentenyl diphosphate, which is not accepted as substrate by the enzyme, exhibits no inhibition on the synthase reaction Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl diphosphate synthase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0054
-
 (2Z,6E,10E)-4-methyl-geranylgeranyl diphosphate potent competitive inhibitor against (2Z,6E,10E)-geranylgeranyl diphosphate, 37°C, pH 37°C Bacillus subtilis
0.0058
-
 (2Z,6E,10E)-4-methyl-geranylgeranyl diphosphate noncompetitive inhibitor against isopentenyl diphosphate, 37°C, pH 37°C Bacillus subtilis
0.008
-
 (2E,6E,10E)-4-methylfarnesyl diphosphate competitive inhibitor against (2Z,6E,10E)-geranylgeranyl diphosphate, 37°C, pH 37°C Bacillus subtilis
0.041
-
 (E)-3-methyl-3-pentenyl diphosphate calculated against isopentenyl diphosphate, 37°C, pH 37°C Bacillus subtilis
0.075
-
 (E)-3-methyl-3-pentenyl diphosphate calculated against (2Z,6E,10E)-geranylgeranyl diphosphate, 37°C, pH 37°C Bacillus subtilis