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Literature summary for 2.5.1.31 extracted from

  • Fujikura, K.; Maki, Y.; Ohya, N.; Satoh, M.; Koyama, T.
    Kinetic studies of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase reaction using 3-desmethyl allylic substrate analogs (2008), Biosci. Biotechnol. Biochem., 72, 851-855.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate mixed inhibition with farnesyl diphosphate, competitive inhibition with reaction intermediate (2Z,6E,10E)-geranylgeranyl diphosphate Micrococcus luteus
3-desmethyl farnesyl diphosphate competitive with farnesyl diphosphate, mixed inhibition with reaction intermediate (2Z,6E,10E)-geranylgeranyl diphosphate; competitive with farnesyl diphosphate, mixed inhibition with reaction intermediate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus
3-desmethyl Z-geranylgeranyl diphosphate mixed inhibition with farnesyl diphosphate, competitive inhibition with reaction intermediate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-
Micrococcus luteus
-
 B-P 26
-
Micrococcus luteus B-P 26
-
-
-
Micrococcus luteus B-P 26
-
 B-P 26
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 ?
-
 ?
additional information artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl Z-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction Micrococcus luteus ?
-
 ?
additional information artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction Micrococcus luteus ?
-
 ?
additional information artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl Z-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction Micrococcus luteus B-P 26 ?
-
 ?
additional information artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate are not used as substrates. The methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. The binding sites for the natural substrate farnesyl diphosphate and those for the intermediate allylic diphosphate, which contains the cis-prenyl unit, are different during the cis-prenyl chain elongation reaction Micrococcus luteus B-P 26 ?
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl diphosphate synthase
-
 Micrococcus luteus
UPP synthase
-
 Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Micrococcus luteus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.017
-
 3-desmethyl Z-geranylgeranyl diphosphate substrate farnesyl diphosphate, pH 7.5, 37°C Micrococcus luteus
0.017
-
 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate substrate farnesyl diphosphate, pH 7.5, 37°C Micrococcus luteus
0.028
-
 3-desmethyl Z-geranylgeranyl diphosphate substrate (Z,E,E)-geranylgeranyl diphosphate, pH 7.5, 37°C Micrococcus luteus
0.028
-
 3-desmethyl (2Z,6E,10E)-geranylgeranyl diphosphate substrate (Z,E,E)-geranylgeranyl diphosphate, pH 7.5, 37°C Micrococcus luteus
0.045
-
 3-desmethyl farnesyl diphosphate substrate farnesyl diphosphate, pH 7.5, 37°C Micrococcus luteus
2.8
-
 3-desmethyl farnesyl diphosphate substrate (Z,E,E)-geranylgeranyl diphosphate, pH 7.5, 37°C Micrococcus luteus
2.8
-
 3-desmethyl farnesyl diphosphate substrate (2Z,6E,10E)-geranylgeranyl diphosphate, pH 7.5, 37°C Micrococcus luteus