Literature summary for 2.5.1.31 extracted from

Kharel, Y.; Takahashi, S.; Yamashita, S.; Koyama, T.
Manipulation of prenyl chain length determination mechanism of cis-prenyltransferases (2006), FEBS J., 273, 647-657.

Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Micrococcus luteus

Protein Variants

Protein Variants	Comment	Organism
A72L	mutation results in shorter ultimate products with C20-35	Micrococcus luteus
A72L/F73L	mutation results in shorter ultimate products with C20-35	Micrococcus luteus
A72L/F73L/W78L	mutation results in shorter ultimate products with C20-35	Micrococcus luteus
F223H	dramatically decreased catalytic activity when farnesyl diphosphate is used as allylic substrate	Micrococcus luteus
F73A	mutation results in shorter ultimate products with C20-35	Micrococcus luteus
F73L	mutation results in shorter ultimate products with C20-35	Micrococcus luteus
W78L	mutation results in shorter ultimate products with C20-35	Micrococcus luteus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0083	-	farnesyl diphosphate	wild type enzyme	Micrococcus luteus
0.0093	-	farnesyl diphosphate	mutant enzyme F73A	Micrococcus luteus
0.014	-	farnesyl diphosphate	mutant enzyme A72L/F73L/W78L	Micrococcus luteus
0.026	-	farnesyl diphosphate	mutant enzyme A72L/F73L	Micrococcus luteus

Organism

Organism	UniProt	Comment	Textmining
Micrococcus luteus	O82827	-	-
Micrococcus luteus B-P 26	O82827	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate + farnesyl diphosphate	-	Micrococcus luteus	C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate	-	?
isopentenyl diphosphate + farnesyl diphosphate	-	Micrococcus luteus B-P 26	C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
undecaprenyl pyrophosphate synthase	-	Micrococcus luteus
UPP, C55 synthase	-	Micrococcus luteus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)