Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | poor activator | Micrococcus luteus | |
deoxycholate | poor activator | Micrococcus luteus | |
lecithin | from egg, stimulates | Micrococcus luteus | |
lecithin | 14fold stimulation at 2 mM | Micrococcus luteus | |
additional information | enzyme is inactive in absence of added effectors | Micrococcus luteus | |
Phospholipid | phospholipid extract from Micrococcus luteus stimulates | Micrococcus luteus | |
Triton X-100 | stimulates | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
(E,E)-farnesyl diphosphate | pH 7.4, 35°C | Micrococcus luteus | |
0.008 | - |
(E,E,E)-geranylgeranyl diphosphate | pH 7.5, 35°C | Micrococcus luteus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Micrococcus luteus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ | Micrococcus luteus | |
Mg2+ | maximal stimulation at 0.1 mM | Micrococcus luteus | |
Mn2+ | enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | 49000 | gel filtration | Micrococcus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004066 | - |
- |
Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
isopentenyl diphosphate + DELTA3-isopentenyl diphosphate | - |
Micrococcus luteus | ? | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | cis,trans-farnesyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | trans,trans-farnesyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranyl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + geranylgeranyl diphosphate | trans,trans,trans-geranylgeranyl diphosphate and cis,trans,trans-geranylgeranyl diphosphate | Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
isopentenyl diphosphate + neryl diphosphate | - |
Micrococcus luteus | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
additional information | reactivities of the allylic substrates increase with chain length: C10, C15, C20. Each new isoprene unit added has a cis-configuration | Micrococcus luteus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Micrococcus luteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: activity maximum, pH 9.0: about 80% of maximal activity | Micrococcus luteus |