BRENDA - Enzyme Database
show all sequences of 2.5.1.31

Purification and characterization of undecaprenylpyrophosphate synthetase

Allen, C.M.; Methods Enzymol. 110, 281-299 (1985)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cardiolipin
Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent
Lactobacillus plantarum
cardiolipin
weak stimulation
Micrococcus luteus
cetyl sulfate
half-maximal activation at 0.04 mM
Lactobacillus plantarum
deoxycholate
weak stimulation
Bacillus subtilis
deoxycholate
half-maximal activation at 0.33 mM
Lactobacillus plantarum
deoxycholate
weak stimulation
Micrococcus luteus
Dodecyl sulfate
half-maximal activation at 0.075 mM
Lactobacillus plantarum
lecithin
weak stimulation
Bacillus subtilis
lecithin
from egg, stimulates
Micrococcus luteus
oleic acid
good activator in absence of detergent
Lactobacillus plantarum
phosphatidic acid
from egg, good activator in absence of detergent
Lactobacillus plantarum
Triton X-100
strong stimulation
Bacillus subtilis
Triton X-100
half-maximal activation at 3.4 mM
Lactobacillus plantarum
Triton X-100
stimulates
Micrococcus luteus
Tween 80
strong stimulation
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-Cyclohexanedione
-
Lactobacillus plantarum
Butanedione
-
Lactobacillus plantarum
iodoacetate
-
Lactobacillus plantarum
additional information
no inhibition by phosphate
Lactobacillus plantarum
Phenylglyoxal
-
Lactobacillus plantarum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00013
-
(E,E)-farnesyl diphosphate
pH 7.5, 35C
Lactobacillus plantarum
0.00192
-
isopentenyl diphosphate
pH 7.5, 35C
Lactobacillus plantarum
0.008
-
(E,E)-farnesyl diphosphate
-
Micrococcus luteus
0.0091
-
(E,E)-farnesyl diphosphate
-
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required
Bacillus subtilis
Co2+
divalent cation required; enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Co2+
divalent cation required; enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
K+
stimulates in presence of Mg2+
Bacillus subtilis
K+
stimulates in presence of Mg2+
Lactobacillus plantarum
Mg2+
best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+; divalent cation required
Bacillus subtilis
Mg2+
divalent cation required; enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Mg2+
divalent cation required; enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
Mn2+
best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+; can partially replace Mg2+
Bacillus subtilis
Mn2+
enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Mn2+
enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
Na+
stimulates in presence of Mg2+
Bacillus subtilis
Na+
stimulates in presence of Mg2+
Lactobacillus plantarum
NH4+
stimulates in presence of Mg2+
Bacillus subtilis
NH4+
stimulates in presence of Mg2+
Lactobacillus plantarum
Zn2+
can partially replace Mg2+
Bacillus subtilis
Zn2+
-
Lactobacillus plantarum
Zn2+
-
Micrococcus luteus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Lactobacillus plantarum
52000
58000
sucrose density gradient centrifugation
Lactobacillus plantarum
53000
60000
gel filtration
Lactobacillus plantarum
56000
-
gel filtration, sucrose density gradient centrifugation
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactobacillus plantarum
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
additional information
Bacillus subtilis
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
additional information
Micrococcus luteus
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Lactobacillus plantarum
-
ATCC 8014
-
Micrococcus luteus
-
-
-
Salmonella newington
-
-
-
Purification (Commentary)
Commentary
Organism
-
Lactobacillus plantarum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Lactobacillus plantarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
637475
Bacillus subtilis
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
637475
Micrococcus luteus
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
isopentenyl diphosphate + farnesyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
-
637475
Salmonella newington
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
trans,trans,trans-geranylgeranyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylneryl diphosphate
cis,trans,trans-geranylneryl diphosphate
637475
Lactobacillus plantarum
?
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Lactobacillus plantarum
?
-
-
-
-
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Bacillus subtilis
?
-
-
-
-
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Micrococcus luteus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Lactobacillus plantarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
and a second optimum at pH 10.2, that is 40% of the optimum at pH 7.5
Lactobacillus plantarum
7.5
-
-
Micrococcus luteus
8.5
-
-
Bacillus subtilis
10.2
-
and a second optimum at pH 7.5 that is higher than the optimum at pH 10.2
Lactobacillus plantarum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cardiolipin
Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent
Lactobacillus plantarum
cardiolipin
weak stimulation
Micrococcus luteus
cetyl sulfate
half-maximal activation at 0.04 mM
Lactobacillus plantarum
deoxycholate
weak stimulation
Bacillus subtilis
deoxycholate
half-maximal activation at 0.33 mM
Lactobacillus plantarum
deoxycholate
weak stimulation
Micrococcus luteus
Dodecyl sulfate
half-maximal activation at 0.075 mM
Lactobacillus plantarum
lecithin
weak stimulation
Bacillus subtilis
lecithin
from egg, stimulates
Micrococcus luteus
oleic acid
good activator in absence of detergent
Lactobacillus plantarum
phosphatidic acid
from egg, good activator in absence of detergent
Lactobacillus plantarum
Triton X-100
strong stimulation
Bacillus subtilis
Triton X-100
half-maximal activation at 3.4 mM
Lactobacillus plantarum
Triton X-100
stimulates
Micrococcus luteus
Tween 80
strong stimulation
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-Cyclohexanedione
-
Lactobacillus plantarum
Butanedione
-
Lactobacillus plantarum
iodoacetate
-
Lactobacillus plantarum
additional information
no inhibition by phosphate
Lactobacillus plantarum
Phenylglyoxal
-
Lactobacillus plantarum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00013
-
(E,E)-farnesyl diphosphate
pH 7.5, 35C
Lactobacillus plantarum
0.00192
-
isopentenyl diphosphate
pH 7.5, 35C
Lactobacillus plantarum
0.008
-
(E,E)-farnesyl diphosphate
-
Micrococcus luteus
0.0091
-
(E,E)-farnesyl diphosphate
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required
Bacillus subtilis
Co2+
divalent cation required; enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Co2+
divalent cation required; enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
K+
stimulates in presence of Mg2+
Bacillus subtilis
K+
stimulates in presence of Mg2+
Lactobacillus plantarum
Mg2+
best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+; divalent cation required
Bacillus subtilis
Mg2+
divalent cation required; enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Mg2+
divalent cation required; enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
Mn2+
best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+; can partially replace Mg2+
Bacillus subtilis
Mn2+
enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+
Lactobacillus plantarum
Mn2+
enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+
Micrococcus luteus
Na+
stimulates in presence of Mg2+
Bacillus subtilis
Na+
stimulates in presence of Mg2+
Lactobacillus plantarum
NH4+
stimulates in presence of Mg2+
Bacillus subtilis
NH4+
stimulates in presence of Mg2+
Lactobacillus plantarum
Zn2+
can partially replace Mg2+
Bacillus subtilis
Zn2+
-
Lactobacillus plantarum
Zn2+
-
Micrococcus luteus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Lactobacillus plantarum
52000
58000
sucrose density gradient centrifugation
Lactobacillus plantarum
53000
60000
gel filtration
Lactobacillus plantarum
56000
-
gel filtration, sucrose density gradient centrifugation
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactobacillus plantarum
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
additional information
Bacillus subtilis
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
additional information
Micrococcus luteus
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactobacillus plantarum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Lactobacillus plantarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
637475
Bacillus subtilis
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
637475
Micrococcus luteus
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
-
-
-
isopentenyl diphosphate + farnesyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
-
637475
Salmonella newington
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + farnesyl diphosphate
trans,trans-farnesyl diphosphate
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranyl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylgeranyl diphosphate
trans,trans,trans-geranylgeranyl diphosphate
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + geranylneryl diphosphate
cis,trans,trans-geranylneryl diphosphate
637475
Lactobacillus plantarum
?
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Lactobacillus plantarum
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Bacillus subtilis
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
isopentenyl diphosphate + neryl diphosphate
-
637475
Micrococcus luteus
C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
-
-
?
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Lactobacillus plantarum
?
-
-
-
-
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Bacillus subtilis
?
-
-
-
-
additional information
involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components
637475
Micrococcus luteus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Lactobacillus plantarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
and a second optimum at pH 10.2, that is 40% of the optimum at pH 7.5
Lactobacillus plantarum
7.5
-
-
Micrococcus luteus
8.5
-
-
Bacillus subtilis
10.2
-
and a second optimum at pH 7.5 that is higher than the optimum at pH 10.2
Lactobacillus plantarum
Other publictions for EC 2.5.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738467
Inokoshi
Inhibition of bacterial undeca ...
Staphylococcus aureus, Staphylococcus aureus MRSA252
J. Antibiot.
2016
1-8
2016
-
-
-
-
-
-
4
-
-
1
-
4
-
5
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
2
4
-
-
-
1
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
738837
Zhu
Antibacterial drug leads: DNA ...
Escherichia coli, Staphylococcus aureus
J. Med. Chem.
58
1215-1227
2015
-
-
-
-
-
-
37
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
32
-
-
-
-
-
-
-
32
37
-
-
-
2
-
-
-
-
-
-
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-
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
739545
Farha
Antagonism screen for inhibito ...
Escherichia coli, Escherichia coli MC1061, Staphylococcus aureus, Staphylococcus aureus USA300
Proc. Natl. Acad. Sci. USA
112
11048-11053
2015
-
-
1
1
-
-
4
-
2
2
-
4
-
5
-
-
1
-
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
1
4
-
-
2
2
-
4
-
-
-
1
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
2
2
-
-
-
739605
Danley
Discovery and structural chara ...
Streptococcus pneumoniae
Protein Sci.
24
20-26
2015
-
-
1
1
-
-
1
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
1
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
737698
Dodbele
Species differences in alterna ...
Bacteroides fragilis, Bacteroides fragilis ATCC 25285, Escherichia coli, Escherichia coli DH5alpha, Vibrio vulnificus, Vibrio vulnificus MO6-24/O
Biochemistry
53
5042-5050
2014
-
-
3
-
-
-
4
-
-
3
-
6
-
11
-
-
3
-
-
-
-
-
20
3
3
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
2
4
-
-
-
3
-
6
-
-
-
3
-
-
-
-
20
3
3
-
-
-
3
-
-
-
-
3
3
-
-
-
722468
Inokoshi
Spirohexalines, new inhibitors ...
Penicillium brasilianum, Penicillium brasilianum FKI-3368
J. Antibiot.
66
37-41
2013
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737459
Lee
-
Reducing the level of undecapr ...
Bacillus subtilis, Bacillus subtilis W168
Antimicrob. Agents Chemother.
57
4267-4275
2013
-
1
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721794
Teng
Structures, mechanisms and inh ...
Escherichia coli, Micrococcus luteus, Micrococcus luteus B-P 26, Streptococcus pneumoniae
Bioorg. Chem.
43
51-57
2012
-
-
-
1
1
-
6
1
-
3
-
4
-
9
-
-
-
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
-
2
6
-
1
-
3
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722551
Inaoka
Undecaprenyl pyrophosphate inv ...
Bacillus subtilis 168, Bacillus subtilis
J. Bacteriol.
194
5632-5637
2012
-
-
-
-
1
-
-
-
-
-
-
-
-
89
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721275
Teng
Fluorescent substrate analog f ...
Escherichia coli
Anal. Biochem.
417
136-141
2011
-
-
-
-
2
-
1
3
-
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
2
-
-
-
-
-
2
-
2
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
2
2
707529
Lee
Biophysical investigation of t ...
Streptococcus pneumoniae
Biochemistry
49
5366-5376
2010
-
-
1
-
-
-
5
-
-
1
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
5
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722345
Rush
Expression of functional bacte ...
Escherichia coli
Glycobiology
20
1585-1593
2010
-
-
1
-
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
685691
Fujikura
Kinetic studies of Micrococcus ...
Micrococcus luteus, Micrococcus luteus B-P 26
Biosci. Biotechnol. Biochem.
72
851-855
2008
-
-
1
-
-
-
3
-
-
-
-
-
-
8
-
-
1
-
-
-
-
-
8
-
1
-
-
-
1
-
-
-
6
-
-
-
-
1
-
-
-
-
-
3
6
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
699031
Kuo
Structure-based inhibitors exh ...
Escherichia coli, Helicobacter pylori
J. Biomed. Biotechnol.
2008
841312
2008
-
1
2
2
2
-
5
8
-
-
-
-
-
7
-
-
2
-
-
-
-
-
3
1
-
-
-
4
2
-
-
-
-
-
3
-
1
4
-
3
3
-
6
9
-
8
-
-
-
-
-
-
-
2
-
-
-
-
3
1
-
-
-
4
2
-
-
-
-
-
-
-
-
-
704720
HKuo
Structure-based inhibitors exh ...
Escherichia coli, Helicobacter pylori
J. Biomed. Biotechnol.
2008
0000
2008
-
-
2
1
1
-
4
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
4
1
-
-
-
-
-
4
-
-
2
-
1
1
-
4
4
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
4
1
-
-
-
-
-
-
-
-
-
676880
Guo
Bisphosphonates target multipl ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
104
10022-10027
2007
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673055
Takahashi
Structure and function of cis- ...
Escherichia coli, Micrococcus luteus, Micrococcus luteus B-P 26
Chem. Rec.
6
194-205
2006
2
-
-
-
-
-
-
-
-
-
1
-
-
12
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673574
Kharel
Manipulation of prenyl chain l ...
Micrococcus luteus, Micrococcus luteus B-P 26
FEBS J.
273
647-657
2006
-
-
1
-
7
-
-
4
-
-
-
-
-
13
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657857
Chen
Substrate and product specific ...
Escherichia coli
Biochem. J.
386
169-176
2005
-
-
1
1
3
-
1
22
-
1
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
13
1
-
-
-
-
-
-
-
-
1
-
1
3
-
-
1
-
22
-
1
-
1
-
-
-
-
-
-
-
-
10
-
1
-
-
13
1
-
-
-
-
-
-
-
-
-
659476
Guo
Crystal structures of undecapr ...
Escherichia coli
J. Biol. Chem.
280
20762-20774
2005
-
-
-
1
4
-
2
24
-
1
-
2
-
3
-
-
-
1
-
-
-
-
4
-
1
-
-
11
1
-
-
-
1
-
-
-
-
-
-
2
8
-
-
3
2
24
-
2
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
11
2
-
-
-
-
-
-
-
6
6
660464
Chang
Substrate binding mode and rea ...
Escherichia coli
Protein Sci.
13
971-978
2004
-
-
-
1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637492
Fujikura
Mutational analysis of allylic ...
Micrococcus luteus, Micrococcus luteus B-P 26
Biochemistry
42
4035-4041
2003
-
-
1
-
8
-
1
46
-
-
-
2
-
18
-
-
-
-
-
-
-
-
12
-
1
-
-
26
1
-
-
-
1
-
-
-
-
2
-
-
16
-
-
2
1
54
-
-
-
2
-
-
-
-
-
-
-
-
12
-
1
-
-
26
1
-
-
-
-
-
-
-
18
18
659084
Khare
Significance of highly conserv ...
Micrococcus luteus, Micrococcus luteus B-P 26
J. Biochem.
134
819-826
2003
-
-
1
-
7
-
-
22
-
1
-
4
-
18
-
-
-
-
-
-
1
-
8
-
1
-
-
13
1
-
-
-
-
-
-
-
-
2
-
-
13
-
-
-
-
23
-
1
-
4
-
-
-
-
-
-
1
-
8
-
2
-
-
13
2
-
-
-
-
-
-
-
1
1
659232
Chang
Catalytic mechanism revealed b ...
Escherichia coli
J. Biol. Chem.
278
29298-29307
2003
1
-
1
1
5
-
-
21
-
3
-
1
-
3
-
-
1
1
-
-
-
-
3
1
1
-
-
15
1
-
-
-
-
-
-
1
-
1
-
1
5
-
-
-
-
21
-
3
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
15
1
-
-
-
-
-
-
-
6
6
707467
Chang
Identification of the active c ...
Escherichia coli
Biochemistry
42
14452-14459
2003
-
-
1
1
6
-
-
9
-
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
9
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
637494
Chen
Probing the conformational cha ...
Escherichia coli
J. Biol. Chem.
277
7369-7376
2002
-
-
1
-
6
-
2
19
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
7
1
-
-
-
2
-
-
-
-
1
-
-
6
-
-
2
2
19
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
7
1
-
-
-
-
-
-
-
7
7
708864
Chen
Synthesis and application of a ...
Escherichia coli
J. Am. Chem. Soc.
124
15217-15224
2002
-
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
637486
Chang
Refolding and characterization ...
Saccharomyces cerevisiae
Protein Expr. Purif.
23
432-439
2001
-
-
1
-
-
-
-
1
1
-
-
1
-
5
-
-
1
-
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
1
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
637489
Fujihashi
Crystal structure of cis-preny ...
Micrococcus luteus, Micrococcus luteus B-P 26
Proc. Natl. Acad. Sci. USA
98
4337-4342
2001
-
-
1
1
-
-
-
-
-
-
1
4
-
17
-
-
1
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637491
Kharel
Identification of significant ...
Micrococcus luteus, Micrococcus luteus B-P 26
J. Biol. Chem.
276
28459-28464
2001
-
-
1
-
9
-
1
58
-
5
-
-
-
11
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
58
-
5
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
637493
Ko
Mechanism of product chain len ...
Escherichia coli
J. Biol. Chem.
276
47474-47482
2001
-
-
-
1
12
-
-
15
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
2
18
-
-
-
-
22
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
7
7
637485
Pan
Product distribution and pre-s ...
Escherichia coli
Biochemistry
39
10936-10942
2000
2
-
1
-
-
-
-
3
-
1
-
1
-
2
-
-
1
-
-
-
2
-
4
-
1
-
-
2
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
3
-
1
-
1
-
-
-
1
-
-
2
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
637487
Fujikura
Significance of Asn-77 and Trp ...
Micrococcus luteus, Micrococcus luteus B-P 26
J. Biochem.
128
917-922
2000
-
-
1
-
6
-
-
33
-
-
-
-
-
11
-
-
1
-
-
-
-
-
4
-
-
-
-
35
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
33
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
35
-
-
-
-
-
-
-
-
-
-
637495
Pan
Effect of site-directed mutage ...
Escherichia coli
Biochemistry
39
13856-13861
2000
-
-
1
-
8
-
-
13
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
13
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
706947
Fujihashi
Crystallization and preliminar ...
Micrococcus luteus, Micrococcus luteus B-P 26
Acta Crystallogr. Sect. D
55
1606-1607
1999
-
-
1
1
-
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708911
Apfel
Use of genomics to identify ba ...
Escherichia coli, Haemophilus influenzae, Streptococcus pneumoniae R6
J. Bacteriol.
181
483-492
1999
-
-
3
-
1
-
1
-
-
1
3
-
-
8
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
-
1
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
637488
Shimizu
Molecular cloning, expression, ...
Escherichia coli, Micrococcus luteus, Micrococcus luteus B-P 26
J. Biol. Chem.
273
19476-19481
1998
-
-
2
-
-
-
-
-
1
-
1
-
-
14
-
-
2
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
2
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708500
Ohnuma
Kinetic studies on the prenyl ...
Bacillus subtilis
FEBS Lett.
257
71-74
1989
-
-
-
-
-
-
4
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
4
5
2
-
-
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637476
Koyama
Undecaprenyl diphosphate synth ...
Micrococcus luteus, Micrococcus luteus B-P 26
J. Biochem.
103
867-871
1988
4
-
-
-
-
-
1
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637482
Fujisaki
Isoprenoid synthesis in Escher ...
Escherichia coli
J. Biochem.
99
1327-1337
1986
1
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
1
-
-
-
-
1
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637475
Allen
Purification and characterizat ...
Bacillus subtilis, Lactobacillus plantarum, Micrococcus luteus, Salmonella newington
Methods Enzymol.
110
281-299
1985
15
-
-
-
-
-
5
4
-
18
4
3
-
4
-
-
1
-
-
-
1
-
20
1
-
-
-
-
4
-
-
-
-
-
-
15
-
-
-
-
-
-
-
5
-
4
-
18
4
3
-
-
-
1
-
-
1
-
20
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
637479
Baba
Photoaffinity labeling of unde ...
Escherichia coli
J. Biol. Chem.
260
10467-10473
1985
-
-
-
-
-
1
-
-
2
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637473
Muth
Undecaprenyl pyrophosphate syn ...
Lactobacillus plantarum
Arch. Biochem. Biophys.
230
49-60
1984
-
-
-
-
-
-
3
3
-
5
2
-
-
3
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
4
-
10
4
-
-
-
-
2
-
-
1
-
2
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
637478
Baba
-
Inactivation of undecaprenylpy ...
Lactobacillus plantarum
Biochemistry
23
1312-1322
1984
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637474
Takahashi
Prenyltransferases of Bacillus ...
Bacillus subtilis
J. Biochem.
92
1527-1537
1982
4
-
-
-
-
-
1
12
-
6
2
-
-
2
-
-
1
-
-
-
1
1
16
-
1
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
12
-
6
2
-
-
-
-
1
-
-
1
1
16
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637481
Baba
Prenyl transferases from Micro ...
Micrococcus luteus
Arch. Biochem. Biophys.
200
474-484
1980
6
-
-
-
-
-
-
2
1
3
1
-
-
2
-
-
1
-
-
-
1
-
8
-
-
-
-
-
1
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
2
1
3
1
-
-
-
-
1
-
-
1
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
637483
Baba
Substrate specificity of undec ...
Lactobacillus plantarum
Biochemistry
17
5598-5604
1978
5
-
-
-
-
-
-
7
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637484
Allen
Lipid activation of undecapren ...
Lactobacillus plantarum
Biochemistry
16
2908-2915
1977
13
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637480
Allen
Lactobacillus plantarum undeca ...
Lactobacillus plantarum
Arch. Biochem. Biophys.
175
236-248
1976
2
-
-
-
-
-
1
-
-
4
1
-
-
2
-
-
1
-
-
-
1
-
4
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
4
1
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
637477
Keenan
Characterization of undecapren ...
Lactobacillus plantarum
Arch. Biochem. Biophys.
161
375-383
1974
4
-
-
-
-
-
2
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-