Protein Variants | Comment | Organism |
---|---|---|
A79F | mutant enzyme subunit-I(wild-type)/subunit-B(A79F). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate | Bacillus subtilis |
A79L | mutant enzyme subunit-I(wild-type)/subunit-B(A79L). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate | Bacillus subtilis |
D97A | mutant enzyme subunit-I(D97A)/subunit-II(wild-type), major products: geranylgeranyl diphosphate, farnesylgeranyl diphosphate, hexaprenyl diphosphate, heptaprenyl diphosphate | Bacillus subtilis |
D97A/A79F | mutant enzyme subunit-I(97A)/subunit-II(A79F) forms exclusively geranylgeranyl diphosphate | Bacillus subtilis |
D97A/A79L | mutant enzyme subunit-I(D97A)/subunit-II(A79L), the final product is farnesylgeranyl diphosphate, increase in the production of geranylgeranyl diphosphate | Bacillus subtilis |
additional information | several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits | Bacillus subtilis |
V76I | mutant enzyme subunit-I(wild-type)/subunit-II(A79L), octaprenyl diphosphate is the final product with farnesyl diphosphate as allylic primer | Bacillus subtilis |
Y103S | mutant enzyme subunit-I(Y103S)/subunit-II(wild-type), final product is octaprenyl diphosphate | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0085 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(A79F) | Bacillus subtilis | |
0.0116 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(I76G) | Bacillus subtilis | |
0.0124 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) | Bacillus subtilis | |
0.0143 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) | Bacillus subtilis | |
0.0168 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0198 | - |
isopentenyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0198 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) | Bacillus subtilis | |
0.0214 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) | Bacillus subtilis | |
0.0259 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) | Bacillus subtilis | |
0.0344 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) | Bacillus subtilis | |
0.0568 | - |
geranylgeranyl diphosphate | pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | - |
Bacillus subtilis | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranyl diphosphate + 4 isopentenyl diphosphate | geranyl diphosphate is a poor substrate | Bacillus subtilis | 4 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | - |
Bacillus subtilis | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HepPP synthase | - |
Bacillus subtilis |