Cloned (Comment) | Organism |
---|---|
to explore the dynamic interaction of the two dissociable components during catalysis, expression vector systems for the two structural genes, gerC1 and gerC3, are constructed separately, and the two components are overproduced in Escherichia coli cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzymatic activity is inhibited by each antiserum against component I or component II, indicating that either component I or component II is confirmed immunochemically to be essential for enzymatic activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 30°C | Bacillus subtilis | |
0.0085 | - |
geranylgeranyl diphosphate | pH 8.5, 30°C | Bacillus subtilis | |
0.0167 | - |
isopentenyl diphosphate | pH 8.5, 30°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requires a divalent cation for the enzymatic activity with an optimal level of 1 mM Mg2+ or 2 mM Mn2+, respectively | Bacillus subtilis | |
Mn2+ | requires a divalent cation for the enzymatic activity with an optimal level of 1 mM Mg2+ or 2 mM Mn2+, respectively | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000 (enzyme component I) + 1 * 36000 (enzyme component II), SDS-PAGE | Bacillus subtilis |
36000 | - |
1 * 29000 (enzyme component I) + 1 * 36000 (enzyme component II), SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Bacillus subtilis | - |
4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2 | - |
Purification (Comment) | Organism |
---|---|
to explore the dynamic interaction of the two dissociable components during catalysis, expression vector systems for the two structural genes, gerC1 and gerC3, are constructed separately, and the two components are overproduced in Escherichia coli cells. Each component is purified homogeneously | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate | enzyme component I, enzyme component II, and farnesyl diphosphate-Mg2+ form a ternary complex during catalysis and that neither isopentenyl diphosphate nor the product, heptaprenyl diphosphate, is included in this complex, which probably represents a catalytically active state of the HepPP synthase. Component I is involved in allylic substrate recognition | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
all-trans-farnesyl diphosphate + 4 isopentenyl diphosphate | enzyme component I, enzyme component II, and farnesyl diphosphate-Mg2+ form a ternary complex during catalysis and that neither isopentenyl diphosphate nor the product, heptaprenyl diphosphate, is included in this complex, which probably represents a catalytically active state of the HepPP synthase. Enzyme component I is involved in allylic substrate recognition | Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 3 isopentenyl diphosphate | - |
Bacillus subtilis | 3 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
additional information | dimethylallyl diphosphate and geranyl diphosphate, are almost inactive as substrates | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 29000 (enzyme component I) + 1 * 36000 (enzyme component II), SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
HepPP synthase | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | recombinant enzyme | Bacillus subtilis |