KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
0.0128 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
0.0133 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, no activation with Mn2+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2 | - |
Purification (Comment) | Organism |
---|---|
partial | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diposphate without accumulation of any prenyl diphosphate shorter than C35 | Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 3 isopentenyl diphosphate | the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diphosphate without accumulation of any prenyl diphosphate shorter than C35. Poor activity of geranylneryl diphosphate contrast with the high activity of the all-trans isomer is consistent with the proposal that this enzyme catalyzes condensations resulting in trans products | Bacillus subtilis | 3 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
additional information | the enzyme does not catalyze a reaction between isopentenyl diphosphate and either dimethylallyl or geranyl diphosphate | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heptaprenyl pyrophosphate synthetase | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Bacillus subtilis |