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Literature summary for 2.5.1.3 extracted from

  • Hayashi, M.; Kobayashi, K.; Esaki, H.; Konno, H.; Akaji, K.; Tazuya, K.; Yamada, K.; Nakabayashi, T.; Nosaka, K.
    Enzymatic and structural characterization of an archaeal thiamin phosphate synthase (2014), Biochim. Biophys. Acta, 1844, 803-809.
    View publication on PubMed
Search for more literature for 2.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum calidifontis

Protein Variants

Protein Variants Comment Organism
H341L no activity Pyrobaculum calidifontis
R320M activity is 1% compared to wild-type enzyme Pyrobaculum calidifontis

Inhibitors

Inhibitors Comment Organism Structure
2-methyl-4-amino-5-hydroxymethylpyrimidine 0.1 mM, 6.3% inhibition Pyrobaculum calidifontis
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate 0.1 mM, 18.2% inhibition Pyrobaculum calidifontis
5-(2-hydroxyethyl)-4-methylthiazole 0.1 mM, 12.3% inhibition Pyrobaculum calidifontis
ATP 0.1 mM, 12.8% inhibition Pyrobaculum calidifontis
diphosphate 0.1 mM, 66% inhibition Pyrobaculum calidifontis
thiamin 0.1 mM, 17.3% inhibition Pyrobaculum calidifontis
thiamin phosphate 0.1 mM, 59% inhibition Pyrobaculum calidifontis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ an absolute requirement of Mg2+ for the activity is replaced to some extent by Co2+, Ca2+ or Zn2+. When Mg2+ is replaced by Mn2+, Ni2+ or Fe2+, however, the activity is much lower or not detected Pyrobaculum calidifontis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole Pyrobaculum calidifontis thiamin biosynthesis diphosphate + thiamine phosphate
-
 ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole Pyrobaculum calidifontis JCM 11548 thiamin biosynthesis diphosphate + thiamine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MSR0
-
-
Pyrobaculum calidifontis JCM 11548 A3MSR0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum calidifontis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of 2.1 nmol thiamin phosphate formed/mg min Pyrobaculum calidifontis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
-
 Pyrobaculum calidifontis diphosphate + thiamine phosphate
-
 ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole thiamin biosynthesis Pyrobaculum calidifontis diphosphate + thiamine phosphate
-
 ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole
-
 Pyrobaculum calidifontis JCM 11548 diphosphate + thiamine phosphate
-
 ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole thiamin biosynthesis Pyrobaculum calidifontis JCM 11548 diphosphate + thiamine phosphate
-
 ?

Synonyms

Synonyms Comment Organism
thiamin phosphate synthase
-
 Pyrobaculum calidifontis
ThiN protein
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
 assay at Pyrobaculum calidifontis
110
-
-
 Pyrobaculum calidifontis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 120 activity increases with an increase in temperature from 80°C to 120°C, and the highest activity is observed around 110°C. The activity at 100°C is about 75% of the highest activity Pyrobaculum calidifontis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 addition of 0.5 M NaCl to the enzyme solution does not improve the thermostability Pyrobaculum calidifontis
100
-
 5 min, 20% loss of activity. 15 min, 50% loss of activity. 2 h, 90% loss of activity Pyrobaculum calidifontis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrobaculum calidifontis
8.5
-
-
 Pyrobaculum calidifontis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 the optimum pH is pH 8.5, and the activity at pH 7.5 is about 60% of that at pH 8.5 Pyrobaculum calidifontis