Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | [Candida] glabrata |
Crystallization (Comment) | Organism |
---|---|
unliganded and in complex with substrates and products, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 0.2-0.25 M MgCl2, and 25-32% PEG400, at 4°C | [Candida] glabrata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains Mg2+ | [Candida] glabrata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] glabrata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | [Candida] glabrata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
[Candida] glabrata | thiamine phosphate + diphosphate | - |
? | |
additional information | the N-terminal domain of the bifunctional enzyme THI6 catalyzes the ligation of the thiamin thiazole and pyrimidine moieties to form thiamine phosphate, and the C-terminal domain catalyzes the phosphorylation of 4-methyl-5-hydroxyethylthiazole in a salvage pathway. Adenosine diphospho-5beta-ethyl-4-methylthiazole-2-carboxylic acid is not a substrate | [Candida] glabrata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
[Candida] glabrata |
Synonyms | Comment | Organism |
---|---|---|
THI6 | bifunctional enzyme | [Candida] glabrata |
thiamin phosphate synthase | - |
[Candida] glabrata |
TPS | - |
[Candida] glabrata |