Cloned (Comment) | Organism |
---|---|
gene erg20 mutants, recombinant expression of N-terminally myc-tagged enzyme, coexpression of the enzyme fused to Cistus creticus 8-hydroxycopalyl diphosphate synthase, CcCLS in Saccharomyces cerevisiae strain AM205 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
F96C | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
F96S | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
additional information | efficient diterpene production in yeast by engineering farnesyl diphosphate synthase Erg20p, EC 2.5.1.10, into a geranylgeranyl diphosphate synthase, overview. Several Erg20p mutants support sclareol yield significantly higher than the yield obtained by overexpression of Cistus creticus GGPPS. A single chromosomally integrated copy of the ERG20(F96C) mutant gene is significantly more efficient than overexpression of a heterologous fungal GGPP synthase in redirecting precursor fluxes towards the diterpene branch | Saccharomyces cerevisiae |
Y95A | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
Y95C/F96H | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
Y95L/F96I | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
Y95S/F96H | site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae | - |
diphosphate + geranylgeranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12051 | gene erg20 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + geranylgeranyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GGPP synthase | - |
Saccharomyces cerevisiae |
GGPPS | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | inefficient substrate channeling between Erg20p and Bts1p results in farnesyl diphosphate accumulation, which in turn leads to feedback inhibition or precursor drain through competing reactions (sterol biosynthesis, dephosphorylation, or other) | Saccharomyces cerevisiae |
metabolism | biosynthesis of sclareol from geranylgeranyl diphosphate, overview | Saccharomyces cerevisiae |
additional information | enzyme structure homology modelling and structure comparisons, overview | Saccharomyces cerevisiae |
physiological function | GGPP synthase catalyzes all three isopentenyl diphosphate additions to dimethylally diphosphate, carrying out in the same active site the reactions catalyzed by both Erg20p (farnesyl diphosphate synthase, EC 2.5.1.10) and Bts1p (geranylgeranyl diphosphate synthase, EC 2.5.1.29) | Saccharomyces cerevisiae |