Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in Picea abies embryogenic tissue | Picea abies |
Protein Variants | Comment | Organism |
---|---|---|
DelP173/DelP174 | decrease in reaction rate | Picea abies |
G257D/P174C | little effect on reation rate, no effect on product composition | Picea abies |
I161M | little effect on reation rate, no effect on product composition | Picea abies |
I235T | decrease in reation rate, single product is geranyldiphosphate | Picea abies |
L180F | little effect on reation rate, product composition is about 98% geranyldiphosphate, 12% geranygeranyl diphosphate | Picea abies |
L180F/P174C | little effect on reation rate, product composition is about 98% geranyldiphosphate, 12% geranygeranyl diphosphate | Picea abies |
L273F | decrease in reation rate, single product is geranyl diphosphate | Picea abies |
M159I | little effect on reation rate, no effect on product composition | Picea abies |
M165C | little effect on reation rate, no effect on product composition | Picea abies |
M165Y | little effect on reation rate, product composition is about 90% geranyldiphosphate, 10% farnesyl diphosphate | Picea abies |
M175I | little effect on reation rate, product composition is about 95% geranyldiphosphate, 3% farnesyl diphosphate, 25% geranygeranyl diphosphate | Picea abies |
M175I/P174C | little effect on reation rate, single product is geranyldiphosphate | Picea abies |
M175I/P174S | little effect on reation rate, no effect on product composition | Picea abies |
P174C | little effect on reation rate, no effect on product composition | Picea abies |
P174S | little effect on reation rate, no effect on product composition | Picea abies |
V227M | decrease in reation rate | Picea abies |
V240L | decrease in reation rate, no effect on product composition | Picea abies |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
dimethylallyl diphosphate | - |
Picea abies |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, activity reaches its highest value at 10 mM MgCl2. At 5 or 15 mM MgCl2, activity is about 65% of maximum | Picea abies |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE of recombinant protein | Picea abies |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | D2IT07 | bifunctional geranyl diphosphate synthase and geranylgeranyl diphosphate synthase | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bark | of sapling | Picea abies | - |
epithelial cell | protein is localized to epithelial cells surrounding the traumatic resin ducts | Picea abies | - |
sapling | expression is restricted to wood and bark | Picea abies | - |
xylem | of sapling | Picea abies | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Picea abies | diphosphate + geranylgeranyl diphosphate | geranyl diphosphate activity of enzyme leads to additional production of geranyl diphosphate. The ratio of geranyl diphosphate to geranylgeranyl diphosphate produced averages approximately 9:1. No synthesis of farnesyl diphosphate | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE of recombinant protein | Picea abies |
Synonyms | Comment | Organism |
---|---|---|
isoprenyl diphosphate synthase | - |
Picea abies |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
dimethylallyl diphosphate | - |
Picea abies |
Organism | Comment | Expression |
---|---|---|
Picea abies | treatment with methyl jasmonate dramatically increases expression level in sapling | up |