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Literature summary for 2.5.1.29 extracted from

  • Lo, C.H.; Chang, Y.H.; Wright, J.D.; Chen, S.H.; Kan, D.; Lim, C.; Liang, P.H.
    Combined experimental and theoretical study of long-range interactions modulating dimerization and activity of yeast geranylgeranyl diphosphate synthase (2009), J. Am. Chem. Soc., 131, 4051-4062.
    View publication on PubMed
Search for more literature for 2.5.1.29

Crystallization (Commentary)

Crystallization (Comment) Organism
the 3D structure of GGPPS reveals an unique positioning of the N-terminal helix A, which protrudes into the other subunit and stabilizes dimerization, although it is far from the main dimer interface. The replacement of residues L8 and I9 at this helix with Gly is sufficient to disrupt the dimer into a monomer, leading to at least 1000fold reduction in activity Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
DELTA 1-17 complete loss of activity, monomer Saccharomyces cerevisiae
DELTA1-6 72% of wild-type activity. Dimer formation Saccharomyces cerevisiae
DELTA1-7 29% of wild-type activity. Dimer and monomer formation Saccharomyces cerevisiae
Delta1-8 0.3% of wild-type activity. Monomer formation Saccharomyces cerevisiae
DELTA1-9 0.2% of wild-type activity. Monomer formation Saccharomyces cerevisiae
E7G 84% of wild-type activity. Dimer and monomer formation Saccharomyces cerevisiae
E7G/L8G 0.6% of wild-type activity Saccharomyces cerevisiae
I9G 2.5% of wild-type activity Saccharomyces cerevisiae
L8G 20% of wild-type activity. Dimer formation Saccharomyces cerevisiae
L8G/I9G 0.3% of wild-type activity. Monomer formation Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 isopentenyl diphosphate mutant E7G/L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0004
-
 isopentenyl diphosphate mutant DELTA1-7, pH 7.5, 25°C Saccharomyces cerevisiae
0.0004
-
 isopentenyl diphosphate mutant E7G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0005
-
 isopentenyl diphosphate mutant DELTA1-8, pH 7.5, 25°C Saccharomyces cerevisiae
0.0005
-
 isopentenyl diphosphate mutant L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0006
-
 isopentenyl diphosphate mutant DELTA1-6, pH 7.5, 25°C Saccharomyces cerevisiae
0.0006
-
 isopentenyl diphosphate mutant L8G/I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0007
-
 isopentenyl diphosphate mutant I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0008
-
 isopentenyl diphosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
0.0014
-
 (E,E)-farnesyl diphosphate mutant DELTA1-7, pH 7.5, 25°C Saccharomyces cerevisiae
0.0014
-
 isopentenyl diphosphate mutant DELTA1-9, pH 7.5, 25°C Saccharomyces cerevisiae
0.0019
-
 (E,E)-farnesyl diphosphate mutant DELTA1-9, pH 7.5, 25°C Saccharomyces cerevisiae
0.0024
-
 (E,E)-farnesyl diphosphate mutant L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0026
-
 (E,E)-farnesyl diphosphate mutant DELTA1-8, pH 7.5, 25°C Saccharomyces cerevisiae
0.0028
-
 (E,E)-farnesyl diphosphate mutant E7G/L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0031
-
 (E,E)-farnesyl diphosphate mutant L8G/I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0032
-
 (E,E)-farnesyl diphosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
0.0036
-
 (E,E)-farnesyl diphosphate mutant I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0043
-
 (E,E)-farnesyl diphosphate mutant DELTA1-6, pH 7.5, 25°C Saccharomyces cerevisiae
0.0044
-
 (E,E)-farnesyl diphosphate mutant E7G, pH 7.5, 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12051
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Saccharomyces cerevisiae diphosphate + geranylgeranyl diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000042
-
 (E,E)-farnesyl diphosphate mutant DELTA1-9, pH 7.5, 25°C Saccharomyces cerevisiae
0.000066
-
 (E,E)-farnesyl diphosphate mutant DELTA1-8, pH 7.5, 25°C Saccharomyces cerevisiae
0.000072
-
 (E,E)-farnesyl diphosphate mutant L8G/I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.00015
-
 (E,E)-farnesyl diphosphate mutant E7G/L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.00062
-
 (E,E)-farnesyl diphosphate mutant I9G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0051
-
 (E,E)-farnesyl diphosphate mutant L8G, pH 7.5, 25°C Saccharomyces cerevisiae
0.0073
-
 (E,E)-farnesyl diphosphate mutant DELTA1-7, pH 7.5, 25°C Saccharomyces cerevisiae
0.018
-
 (E,E)-farnesyl diphosphate mutant DELTA1-6, pH 7.5, 25°C Saccharomyces cerevisiae
0.021
-
 (E,E)-farnesyl diphosphate mutant E7G, pH 7.5, 25°C Saccharomyces cerevisiae
0.025
-
 (E,E)-farnesyl diphosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae