Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.29 extracted from

  • Lütke Brinkhaus, F.; Rilling, H.C.
    Purification of geranylgeranyl diphosphate synthase from Phycomyces blakesleanus (1988), Arch. Biochem. Biophys., 266, 607-612.
    View publication on PubMed
Search for more literature for 2.5.1.29

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 isopentenyl diphosphate
-
 Phycomyces blakesleeanus
0.06
-
 farnesyl diphosphate
-
 Phycomyces blakesleeanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE Phycomyces blakesleeanus
60000
-
 gel filtration, enzyme consists of 2 identical subunits of 30000 Da with a tendency to dissociate Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Phycomyces blakesleeanus preferred allylic substrate geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Phycomyces blakesleeanus less than 5% of the activity with farnesyl diphosphate geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate Phycomyces blakesleeanus less than 5% of the activity with farnesyl diphosphate geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol, DE-52 cellulose, DEAE Trisacryl-M, Reactive blue 4-agarose, Reactive red 120-agarose Phycomyces blakesleeanus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
-
 Phycomyces blakesleeanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate preferred allylic substrate Phycomyces blakesleeanus geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate less than 5% of the activity with farnesyl diphosphate Phycomyces blakesleeanus geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate less than 5% of the activity with farnesyl diphosphate Phycomyces blakesleeanus geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE Phycomyces blakesleeanus