Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 10 mM, 97% inhibition | Methanothermobacter thermautotrophicus | |
p-chloromercuribenzoate | 1 mM, 90% inhibition, 30% inhibition in the presence of 800 mM KCl | Methanothermobacter thermautotrophicus | |
SDS | 1%, 97% inhibition | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0126 | - |
geranyl diphosphate | - |
Methanothermobacter thermautotrophicus | |
0.0147 | - |
farnesyl diphosphate | - |
Methanothermobacter thermautotrophicus | |
0.0168 | - |
dimethylallyl diphosphate | - |
Methanothermobacter thermautotrophicus | |
0.0308 | - |
isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 800 mM, activates | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
native PAGE | Methanothermobacter thermautotrophicus |
78000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Methanothermobacter thermautotrophicus | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Methanothermobacter thermautotrophicus SF-4 | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Methanothermobacter thermautotrophicus | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Methanothermobacter thermautotrophicus SF-4 | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Methanothermobacter thermautotrophicus | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Methanothermobacter thermautotrophicus SF-4 | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
SF-4 | - |
Methanothermobacter thermautotrophicus SF-4 | - |
SF-4 | - |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
with farnesyl diphosphate | Methanothermobacter thermautotrophicus |
0.289 | - |
with geranyl diphosphate | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | geranylgeranyl diphosphate + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus SF-4 | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus SF-4 | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus | geranylgeranyl diphosphate + 2 diphosphate | geranyl diphosphate + farnesyl diphosphate, ratio of farnesyl diphosphate to geranylgeranyl diphosphate increases to more than 2 under both conditions of substrate and product inhibition | ? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus SF-4 | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Methanothermobacter thermautotrophicus SF-4 | geranylgeranyl diphosphate + 2 diphosphate | geranyl diphosphate + farnesyl diphosphate, ratio of farnesyl diphosphate to geranylgeranyl diphosphate increases to more than 2 under both conditions of substrate and product inhibition | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Methanothermobacter thermautotrophicus |
65 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 72 | approx. 30% of maximal activity at 30°C, approx. 80% of maximal activity at 72°C | Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
in presence of 800 mM KCl, 30 min, stable | Methanothermobacter thermautotrophicus |
72 | - |
in the presence of 3500 mM KCl | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Methanothermobacter thermautotrophicus |