BRENDA - Enzyme Database show
show all sequences of 2.5.1.27

Ectopic expression of KNOTTED1-like homeobox protein induces expression of cytokinin biosynthesis genes in rice

Sakamoto, T.; Sakakibara, H.; Kojima, M.; Yamamoto, Y.; Nagasaki, H.; Inukai, Y.; Sato, Y.; Matsuoka, M.; Plant Physiol. 142, 54-62 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expressed in Escherichia coli
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0051
-
ATP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.007
-
ATP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.0087
-
dimethylallyl diphosphate
in the presence of 0.2 mM ADP
Oryza sativa
0.0147
-
ADP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.0207
-
dimethylallyl diphosphate
in the presence of 0.2 mM ADP
Oryza sativa
0.0298
-
ADP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.147
-
AMP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.414
-
AMP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
Q33CD8
-
-
Oryza sativa
Q33CE0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + ADP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + AMP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + ATP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
;
Oryza sativa
AMP
;
Oryza sativa
ATP
;
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Oryza sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Oryza sativa
AMP
-
Oryza sativa
ATP
-
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0051
-
ATP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.007
-
ATP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.0087
-
dimethylallyl diphosphate
in the presence of 0.2 mM ADP
Oryza sativa
0.0147
-
ADP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.0207
-
dimethylallyl diphosphate
in the presence of 0.2 mM ADP
Oryza sativa
0.0298
-
ADP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.147
-
AMP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
0.414
-
AMP
in the presence of 0.2 mM dimethylallyl diphosphate
Oryza sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + ADP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + AMP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + ATP
-
676617
Oryza sativa
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
Other publictions for EC 2.5.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739429
Frebortova
Biochemical characterization o ...
Nostoc sp.
PLoS ONE
10
e0138468
2015
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2
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721952
Yeh
-
Molecular characterization of ...
Bambusa oldhamii
Bot. Stud.
52
249-256
2011
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723389
Qin
Regulated expression of an iso ...
Arachis hypogaea
Plant Cell Physiol.
52
1904-1914
2011
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705984
Chu
Crystal structure and substrat ...
Humulus lupulus
Nucleic Acids Res.
38
1738-1748
2010
-
-
1
1
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2
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5
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2
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1
1
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721466
Wagiran
-
Agrobacterium tumefaciens-medi ...
Oryza sativa
Aust. J. Crop Sci.
4
421-429
2010
-
-
1
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1
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722266
Chu
Binding and catalysis of Humul ...
Humulus lupulus
FEBS Lett.
584
4083-4088
2010
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3
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7
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2
2
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722898
Zhang
Senescence-inducible expressio ...
Manihot esculenta
J. Integr. Plant Biol.
52
653-669
2010
-
-
1
-
1
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1
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3
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2
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1
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2
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1
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-
1
2
2
1
-
-
688633
Ma
-
Expression of isopentenyl tran ...
Agrobacterium tumefaciens
J. Plant Growth Regul.
27
68-76
2008
-
-
1
-
1
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1
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689564
Brugiere
A member of the maize isopente ...
Zea mays
Plant Mol. Biol.
67
215-229
2008
-
-
1
-
1
-
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3
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6
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4
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6
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4
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689799
Sugawara
Structural insight into the re ...
Agrobacterium tumefaciens
Proc. Natl. Acad. Sci. USA
105
2734-2739
2008
-
-
-
1
5
-
-
13
-
1
-
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-
5
-
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-
1
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2
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-
11
-
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1
5
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13
-
1
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2
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11
-
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-
706291
Galichet
Farnesylation directs AtIPT3 s ...
Arabidopsis thaliana
Plant Physiol.
146
1155-1164
2008
-
-
1
-
-
-
-
-
3
-
-
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-
3
-
1
1
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-
1
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1
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671796
Abe
Enzymatic formation of unnatur ...
Morus alba
Biochem. Biophys. Res. Commun.
355
795-800
2007
-
-
1
-
10
-
-
2
-
2
1
-
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3
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1
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1
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12
-
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2
1
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3
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1
3
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10
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2
-
2
1
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1
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1
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-
12
-
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2
1
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676481
Ballester
Efficient production of transg ...
Citrus sinensis, Citrus sinensis x Citrus trifoliata
Plant Cell Rep.
26
39-45
2007
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676513
Zhang
Over-expression of SOB5 sugges ...
Agrobacterium tumefaciens
Plant J.
46
834-848
2006
-
-
1
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4
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676617
Sakamoto
Ectopic expression of KNOTTED1 ...
Oryza sativa
Plant Physiol.
142
54-62
2006
-
-
1
-
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-
-
8
-
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5
-
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6
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3
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2
6
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8
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6
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676669
Ye
Identification and characteriz ...
Glycine max
Plant Sci.
170
542-550
2006
-
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660393
Sakakibara
Agrobacterium tumefaciens incr ...
Agrobacterium tumefaciens, Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
102
9972-9977
2005
-
-
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3
2
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2
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5
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676399
He
-
Cell cycle specific isopenteny ...
Agrobacterium tumefaciens
Plant Biotechnol.
22
261-270
2005
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1
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660100
Sakano
Molecular cloning, expression, ...
Humulus lupulus
Phytochemistry
65
2439-2446
2004
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1
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4
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1
1
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5
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1
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2
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4
1
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4
1
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1
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4
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1
1
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1
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2
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4
1
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4
1
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660174
Miyawaki
Expression of cytokinin biosyn ...
Arabidopsis thaliana
Plant J.
37
128-138
2004
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1
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6
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10
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1
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1
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10
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1
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659797
Kakimoto
Biosynthesis of cytokinins ...
Agrobacterium tumefaciens, Dictyostelium discoideum
J. Plant Res.
116
233-239
2003
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2
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2
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659798
Takei
A new method for enzymatic pre ...
Arabidopsis thaliana
J. Plant Res.
116
259-263
2003
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1
1
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1
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2
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489901
Takei
Identification of genes encodi ...
Arabidopsis thaliana
J. Biol. Chem.
276
26405-26410
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