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Literature summary for 2.5.1.23 extracted from

  • Cacciapuoti, G.; Porcelli, M.; Carteni-Farina, M.; Gambacorta, A.; Zappia, V.
    Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium (1986), Eur. J. Biochem., 161, 263-271.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5'-isobutylthioadenosine
-
Saccharolobus solfataricus
5'-methylthiotubercidin
-
Saccharolobus solfataricus
5'-S-ethyl-5'-thioadenosine
-
Saccharolobus solfataricus
5'-S-methyl-5'-thioadenosine powerful competitive inhibitor Saccharolobus solfataricus
A9154C
-
Saccharolobus solfataricus
n-propylthioadenosine
-
Saccharolobus solfataricus
S-adenosyl(5')-3-thiopropylamine
-
Saccharolobus solfataricus
S-adenosyl-3-thio-1,8-diaminooctane
-
Saccharolobus solfataricus
S-adenosyl-3-thiopropylamine
-
Saccharolobus solfataricus
sinefungin
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0079
-
S-adenosylmethioninamine pH 7.5, 78°C Saccharolobus solfataricus
0.954
-
sym-norspermidine pH 7.5, 78°C Saccharolobus solfataricus
1.539
-
spermidine pH 7.5, 78°C Saccharolobus solfataricus
1.675
-
propane-1,3-diamine pH 7.5, 78°C Saccharolobus solfataricus
3.85
-
putrescine pH 7.5, 78°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol no activity is associated with the cellular particulate fraction Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
3 * 35000, SDS-PAGE Saccharolobus solfataricus
110000
-
gel filtration, ultracentrifugation analysis Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.059
-
pH 7.5, 78°C Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, pH 7.5, stable for 1 months Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosylmethioninamine + propane-1,3-diamine
-
Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
ir
S-adenosylmethioninamine + propane-1,3-diamine
-
Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
ir
S-adenosylmethioninamine + putrescine 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + spermidine
-
ir
S-adenosylmethioninamine + putrescine 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + spermidine
-
ir
S-adenosylmethioninamine + spermidine 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + spermine
-
ir
S-adenosylmethioninamine + spermidine 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + spermine
-
ir
S-adenosylmethioninamine + sym-norspermidine 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + ?
-
ir
S-adenosylmethioninamine + sym-norspermidine 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + ?
-
ir

Subunits

Subunits Comment Organism
trimer 3 * 35000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
78
-
assay at Saccharolobus solfataricus
90
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 110 50°C: about 40% of maximal activity, 110°C: about 45% of maximal activity, 120°C: about 20% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
1 h, completely stable Saccharolobus solfataricus
120
-
no protection by substrate against thermal inactivation at 120°C is observed by incubating the enzyme in the presence of polyamines Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6: about 50% of maximal activity, pH 9.5: about 50% of maximal activity Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0037
-
5'-S-methyl-5'-thioadenosine pH 7.5, 78°C Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
5.3

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.014
-
pH 7.5, 78°C Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine
0.016
-
pH 7.5, 78°C Saccharolobus solfataricus S-adenosyl-3-thiopropylamine
0.021
-
pH 7.5, 78°C Saccharolobus solfataricus S-adenosyl-3-thio-1,8-diaminooctane
0.1
-
pH 7.5, 78°C Saccharolobus solfataricus sinefungin
0.1
-
pH 7.5, 78°C Saccharolobus solfataricus 5'-S-ethyl-5'-thioadenosine
0.1
-
pH 7.5, 78°C Saccharolobus solfataricus A9154C
0.12
-
pH 7.5, 78°C Saccharolobus solfataricus n-propylthioadenosine
0.15
-
pH 7.5, 78°C Saccharolobus solfataricus 5'-isobutylthioadenosine

Expression

Organism Comment Expression
Saccharolobus solfataricus no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium additional information