Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthioadenosine | strong | Bos taurus | |
additional information | no inhibition by erythro-9-(2-hydroxy-3-nonyl)adenine, methylglyoxal-bis-(guanylhydrazone), aminoguanidine, guanethidine, L-canaline, MgCl2, KCl, diamines, polyamines, polyamine derivatives | Bos taurus | |
N-ethylmaleimide | in the absence of sulfhydryl compounds | Bos taurus | |
PCMB | in the absence of sulfhydryl compounds | Bos taurus | |
putrescine | - |
Bos taurus | |
S-adenosyl-L-homocysteine | not | Bos taurus | |
S-adenosyl-L-methionine | not | Bos taurus | |
spermine | product inhibition, weak | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
S-adenosylmethioninamine | - |
Bos taurus | |
0.006 | - |
spermidine | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Bos taurus |
88000 | 90000 | brain, gel filtration, sedimentation equilibrium centrifugation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + spermidine | Bos taurus | involved in polyamine biosynthetic pathway | 5'-methylthioadenosine + spermine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
spermine-Sepharose affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.396 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | overview | Bos taurus | ? | - |
? | |
S-adenosylmethioninamine + spermidine | strictly specific for spermidine | Bos taurus | 5'-methylthioadenosine + spermine | - |
? | |
S-adenosylmethioninamine + spermidine | involved in polyamine biosynthetic pathway | Bos taurus | 5'-methylthioadenosine + spermine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.1, brain | Bos taurus |
7 | 8 | broad | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor requirement | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
5'-methylthioadenosine | - |
Bos taurus | |
0.0017 | - |
putrescine | - |
Bos taurus |