Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.21 extracted from

  • Hong, W.K.; Heo, S.Y.; Park, H.M.; Kim, C.H.; Sohn, J.H.; Kondo, A.; Seo, J.W.
    Characterization of a squalene synthase from the thraustochytrid microalga Aurantiochytrium sp. KRS101 (2013), J. Microbiol. Biotechnol., 23, 759-765.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene krsSQS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain BL21(DE3) Aurantiochytrium sp. KRS101

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Aurantiochytrium sp. KRS101 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aurantiochytrium sp. KRS101

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Aurantiochytrium sp. KRS101
-
squalene + 2 diphosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Aurantiochytrium sp. KRS101 U3KZM8 gene krsSQS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aurantiochytrium sp. KRS101

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
Aurantiochytrium sp. KRS101 squalene + 2 diphosphate + NADP+
-
?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ the enzyme initially catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to form presqualene diphosphate (PSPP), and next converting PSPP to squalene in a reaction requiring NADPH and Mg2+ Aurantiochytrium sp. KRS101 squalene + 2 diphosphate + NADP+ product analysis by GC-MS analysis ?

Subunits

Subunits Comment Organism
? x * 42700-43300, recombinant His6-tagged enzyme, SDS-PAGE Aurantiochytrium sp. KRS101

Synonyms

Synonyms Comment Organism
SQS
-
Aurantiochytrium sp. KRS101

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aurantiochytrium sp. KRS101

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Aurantiochytrium sp. KRS101

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Aurantiochytrium sp. KRS101

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first dedicated step in the biosynthesis of sterols and other triterpenoids Aurantiochytrium sp. KRS101
physiological function enzyme SQS plays an important role in regulating isoprenoid biosynthesis in eukaryotes Aurantiochytrium sp. KRS101