Cloned (Comment) | Organism |
---|---|
gene krsSQS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Aurantiochytrium sp. KRS101 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Aurantiochytrium sp. KRS101 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aurantiochytrium sp. KRS101 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Aurantiochytrium sp. KRS101 | - |
squalene + 2 diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aurantiochytrium sp. KRS101 | U3KZM8 | gene krsSQS | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aurantiochytrium sp. KRS101 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Aurantiochytrium sp. KRS101 | squalene + 2 diphosphate + NADP+ | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | the enzyme initially catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to form presqualene diphosphate (PSPP), and next converting PSPP to squalene in a reaction requiring NADPH and Mg2+ | Aurantiochytrium sp. KRS101 | squalene + 2 diphosphate + NADP+ | product analysis by GC-MS analysis | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42700-43300, recombinant His6-tagged enzyme, SDS-PAGE | Aurantiochytrium sp. KRS101 |
Synonyms | Comment | Organism |
---|---|---|
SQS | - |
Aurantiochytrium sp. KRS101 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aurantiochytrium sp. KRS101 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Aurantiochytrium sp. KRS101 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Aurantiochytrium sp. KRS101 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first dedicated step in the biosynthesis of sterols and other triterpenoids | Aurantiochytrium sp. KRS101 |
physiological function | enzyme SQS plays an important role in regulating isoprenoid biosynthesis in eukaryotes | Aurantiochytrium sp. KRS101 |