Cloned (Comment) | Organism |
---|---|
genes WsSQS1and WsSQS2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR tissue expression analysis, expression as soluble recombinant enzymes with 24 hydrophobic amino acids deleted from the C-terminus and expressed as His6-tagged proteins in Escherichia coli | Withania somnifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, the catalytic site is composed of the large central cavity formed by antiparallel alpha helices with two aspartate rich regions (DXXXD) on opposite walls, these residues are considered to play roles in binding of prenyl phosphates by binding Mg2+ ions | Withania somnifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, recombinant C-terminally truncated, His6-tagged WsSQS1and WsSQS2, SDS-PAGE | Withania somnifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate | Withania somnifera | - |
diphosphate + presqualene diphosphate | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Withania somnifera | overall reaction | squalene + 2 diphosphate + NADP+ | - |
? | |
presqualene diphosphate + NADPH + H+ | Withania somnifera | - |
squalene + diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | H9L9T8 | WsSQS; genes WsSQS1and WsSQS2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged WsSQS1and WsSQS2 proteins from Escherichia coli by nickel affinity chromatgraphy | Withania somnifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | higher SQS comtent in younger than in mature leaves | Withania somnifera | - |
additional information | quantitative real-time PCR tissue expression analysis, overview. WsSQS expresses more in young leaves than mature leaves, stem and root | Withania somnifera | - |
root | - |
Withania somnifera | - |
stem | - |
Withania somnifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate | - |
Withania somnifera | diphosphate + presqualene diphosphate | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | overall reaction | Withania somnifera | squalene + 2 diphosphate + NADP+ | - |
? | |
presqualene diphosphate + NADPH + H+ | - |
Withania somnifera | squalene + diphosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant C-terminally truncated, His6-tagged WsSQS1and WsSQS2, SDS-PAGE | Withania somnifera |
Synonyms | Comment | Organism |
---|---|---|
SQS | - |
Withania somnifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Withania somnifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Withania somnifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Withania somnifera |
General Information | Comment | Organism |
---|---|---|
metabolism | squalene synthase is a key enzyme in the regulation of isoprenoid biosynthesis and is important in the withanolides biosynthesis pathway, overview | Withania somnifera |
additional information | the catalytic site is composed of the large central cavity formed by antiparallel alpha helices with two aspartate rich regions (DXXXD) on opposite walls, these residues are considered to play roles in binding of prenyl phosphates by binding Mg2+ ions | Withania somnifera |