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Literature summary for 2.5.1.21 extracted from

  • Sasiak, K.; Rilling, H.C.
    Purification to homogeneity and some properties of squalene synthetase (1988), Arch. Biochem. Biophys., 260, 622-627.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
farnesyl diphosphate substrate inhibitor of squalene synthase reaction Saccharomyces cerevisiae
NADP+ inhibitor of squalene synthesis Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADPH
-
Saccharomyces cerevisiae
0.004
-
NADH
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.816
-
presqualene Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + NADH + H+
-
Saccharomyces cerevisiae squalene + diphosphate + NAD+
-
?
farnesyl diphosphate + farnesyl diphosphate
-
Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
farnesyl diphosphate + NAD(P)H
-
Saccharomyces cerevisiae squalene + diphosphate + NAD(P)+ presqualene diphosphate and squalene are produced in a ratio of 6:1 ?
presqualene diphosphate + NAD(P)H one protein with 2 catalytic sites may be involved in synthesis of presqualene diphosphate and for its reduction to squalene Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Saccharomyces cerevisiae