Application | Comment | Organism |
---|---|---|
molecular biology | model for rubber biosynthesis | Taraxacum kok-saghyz |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae SNH23-D7 (temperature sensitive mutant strain for dehydrodoichyl diphosphate synthase) or Nicotiana tabacum protoplasts, enzyme remains fully functional, and expression in Escherichai coli BL21 for sequencing and Western blot analysis with antibodies | Taraxacum kok-saghyz |
Protein Variants | Comment | Organism |
---|---|---|
additional information | complementation of enzyme deficient Saccharomyces by each Taraxacum kok-saghyz rubber transferase enzyme, protein extracts from protoplasts expressing recombinant enzyme show high activities of each isozyme | Taraxacum kok-saghyz |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 to 5 mM MgCl2 increase 2-methyl-1,3-butadiene incorporation, optimum 4-5 mM, with 7.5 mM decline below the 2 mM value, almost loss of activity with 10 mM MgCl2 | Taraxacum kok-saghyz | |
Mg2+ | increase in in 2-methyl-1,3-butadiene incorporation from 2 up to 5 mM MgCl2, optimum 4-5 mM, with 7.5 mM decline below the 2 mM value, almost loss of activity with 10 mM MgCl2 | Taraxacum kok-saghyz |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE, TkCPT1 | Taraxacum kok-saghyz |
32000 | - |
SDS-PAGE, TkCPT2 | Taraxacum kok-saghyz |
34000 | - |
SDS-PAGE, TkCPT3 | Taraxacum kok-saghyz |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Taraxacum kok-saghyz | natural rubber is synthesized by adding activated 2-methyl-1,3-butadiene to the growing chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taraxacum kok-saghyz | - |
Russian dandelion | - |
Purification (Comment) | Organism |
---|---|
purification of rubber particles containing the enzyme by harvesting latex from petioles of roots, separating latex fractions by centrifugation with extraction buffer (100 mM Tris-HCl, pH 7.8, 350 mM sorbitol, 10 mM NaCl, 5 mM MgCl2, 5 mM DTT), rubber phase is further centrifuged, washed with buffer and used for enzyme assays | Taraxacum kok-saghyz |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Taraxacum kok-saghyz | - |
additional information | only minimally expressed in leaves, pedicels, and roots | Taraxacum kok-saghyz | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000026 | - |
2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h | Taraxacum kok-saghyz |
0.000003 | - |
2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h | Taraxacum kok-saghyz |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate + (E,E)-farnesyl diphosphate + cis-1,4-polyisoprene | - |
Taraxacum kok-saghyz | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
additional information | natural rubber is synthesized by adding activated 2-methyl-1,3-butadiene to the growing chain | Taraxacum kok-saghyz | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cis-1,4-polyprenylcistransferase | - |
Taraxacum kok-saghyz |
cis-prenyltransferase | - |
Taraxacum kok-saghyz |
rubber transferase | - |
Taraxacum kok-saghyz |
TkCPT1 | - |
Taraxacum kok-saghyz |
TkCPT2 | - |
Taraxacum kok-saghyz |
TkCPT3 | - |
Taraxacum kok-saghyz |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme produces very pure poly(cis-1,4-isoprene) and retains its capacity to produce natural rubber ex vivo | Taraxacum kok-saghyz |