Literature summary for 2.5.1.19 extracted from

Tian, Y.S.; Xu, J.; Peng, R.H.; Xiong, A.S.; Xu, H.; Zhao, W.; Fu, X.Y.; Han, H.J.; Yao, Q.H.
Mutation by DNA shuffling of 5-enolpyruvylshikimate-3-phosphate synthase from Malus domestica for improved glyphosate resistance (2013), Plant Biotechnol. J., 11, 829-838.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells and in Oryza sativa	Malus domestica

Protein Variants

Protein Variants	Comment	Organism
A187T	the mutation alters glyphosate resistance and slightly reduces enzyme activity	Malus domestica
T101A	the mutation alters glyphosate resistance and strongly reduces enzyme activity	Malus domestica
T101A/A187T	the mutation alters glyphosate resistance and reduces enzyme activity	Malus domestica

Inhibitors

Inhibitors	Comment	Organism	Structure
glyphosate	-	Malus domestica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	phosphoenolpyruvate	wild type enzyme, at 28°C, pH not specified in the publication	Malus domestica
0.032	-	phosphoenolpyruvate	mutant enzyme A187T, at 28°C, pH not specified in the publication	Malus domestica
0.049	-	phosphoenolpyruvate	mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication	Malus domestica
0.081	-	phosphoenolpyruvate	mutant enzyme T101A, at 28°C, pH not specified in the publication	Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	Malus domestica	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA agarose column chromatography	Malus domestica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Malus domestica	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?

Synonyms

Synonyms	Comment	Organism
5-enolpyruvylshikimate-3-phosphate synthase	-	Malus domestica
EPSPS	-	Malus domestica

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000048	-	glyphosate	wild type enzyme, at 28°C, pH not specified in the publication	Malus domestica
0.00048	-	glyphosate	mutant enzyme A187T, at 28°C, pH not specified in the publication	Malus domestica
0.0092	-	glyphosate	mutant enzyme T101A, at 28°C, pH not specified in the publication	Malus domestica
0.48	-	glyphosate	mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication	Malus domestica

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00093	-	wild type enzyme, at 28°C, pH not specified in the publication	Malus domestica	glyphosate
0.0084	-	mutant enzyme A187T, at 28°C, pH not specified in the publication	Malus domestica	glyphosate
0.27	-	mutant enzyme T101A, at 28°C, pH not specified in the publication	Malus domestica	glyphosate
55	-	mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication	Malus domestica	glyphosate

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Release 2023.1 (January 2023)