Literature summary for 2.5.1.19 extracted from

Majumder, K.; Selvapandiyan, A.; Fattah, F.A.; Arora, N.; Ahmad, S.; Bhatnagar, R.K.
5-Enolpyruvylshikimate-3-phosphate synthase of Bacillus subtilis is an allosteric enzyme. Analysis of Arg24 -> Asp, Pro105 -> Ser and His385 -> Lys mutations suggests a hidden phosphoenolpyruvate-binding site (1995), Eur. J. Biochem., 229, 99-106.

Search for more literature for 2.5.1.19

Activating Compound

Activating Compound	Comment	Organism	Structure
NH4Cl	maximal activation at 100 mM	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and R24D, P105S, H385L mutant enzymes in Escherichia coli	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
H385L	0.2% of wild-type activity, 2fold activation at 100 mM NH4Cl	Bacillus subtilis
H385N	6% of wild-type activity	Bacillus subtilis
P105S	69% of wild-type activity, 8fold activation at 100 mM NH4Cl	Bacillus subtilis
R24D	0.8% of wild-type activity, 2fold activation at 100 mM NH4Cl	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
N-phosphonomethylglycine	0.25 mM, 50% inhibition of NH4Cl activated enzyme, 75 mM, 50% inhibition of nonactivated enzyme	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	phosphoenolpyruvate	wild-type enzyme	Bacillus subtilis
0.16	-	shikimate 3-phosphate	H385 mutant enzyme	Bacillus subtilis
0.2	-	phosphoenolpyruvate	R24D mutant enzyme	Bacillus subtilis
0.2	-	shikimate 3-phosphate	R24D mutant enzyme	Bacillus subtilis
0.33	-	shikimate 3-phosphate	wild-type enzyme	Bacillus subtilis
0.43	-	shikimate 3-phosphate	P105S mutant enzyme	Bacillus subtilis
0.59	-	phosphoenolpyruvate	P105S mutant enzyme	Bacillus subtilis
1	-	phosphoenolpyruvate	H385L mutant enzyme	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	mixture of monomers and dimers in the absence of NH4Cl, only monomers in the presence of NH4Cl, gel filtration	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	Bacillus subtilis	involved in aromatic acid biosynthesis	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, ammonium sulfate	Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.4	-	-	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Bacillus subtilis	phosphate + 5-enolpyruvylshikimate 3-phosphate	i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate	r
phosphoenolpyruvate + 3-phosphoshikimate	involved in aromatic acid biosynthesis	Bacillus subtilis	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.04	-	N-phosphonomethylglycine	H385L mutant enzyme	Bacillus subtilis
0.05	-	N-phosphonomethylglycine	R24D mutant enzyme	Bacillus subtilis
0.06	-	N-phosphonomethylglycine	wild-type enzyme	Bacillus subtilis
0.21	-	N-phosphonomethylglycine	P105S mutant enzyme	Bacillus subtilis

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Release 2023.1 (January 2023)