Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bromosulfophthalein | - |
Schistocerca gregaria | |
Cibacron blue | most potent inhibitor | Schistocerca gregaria | |
delphinidin chloride | strong inhibition | Schistocerca gregaria | |
Ethacrynic acid | - |
Schistocerca gregaria | |
additional information | not inhibited by 18alpha-glycyrrhetinic acid and cyanidin | Schistocerca gregaria | |
quercetin | strong inhibition | Schistocerca gregaria | |
rutin | - |
Schistocerca gregaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
1-chloro-2,4-dinitrobenzene | at pH 8.0 and 30°C | Schistocerca gregaria | |
0.354 | - |
glutathione | at pH 8.0 and 30°C | Schistocerca gregaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistocerca gregaria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and GSH-Sepharose column chromatography | Schistocerca gregaria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | highest enzyme activity in fat body | Schistocerca gregaria | - |
foregut | - |
Schistocerca gregaria | - |
hindgut | - |
Schistocerca gregaria | - |
midgut | - |
Schistocerca gregaria | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.66 | - |
crude extract, at pH 8.0 and 30°C | Schistocerca gregaria |
42.8 | - |
after 16.1fold purification, at pH 8.0 and 30°C | Schistocerca gregaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Schistocerca gregaria | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Schistocerca gregaria | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + cumene hydroperoxide | 114% activity compared to 1-chloro-2,4-dinitrobenzene | Schistocerca gregaria | ? | - |
? | |
additional information | the enzyme has no detectable activity toward 1,2-dichloro-4-nitrobenzene, ethacrynic acid, bromosulfophthalein, 1,2-epoxy-3-(4-nitrophenoxy) propane, 4-nitrophenethyl bromide and styrene oxide | Schistocerca gregaria | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Schistocerca gregaria |
? | x * 23600, calculated from amino acid sequence | Schistocerca gregaria |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Schistocerca gregaria |
GST | - |
Schistocerca gregaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
optimum at pH 8.0 followed by an approximately 70% decrease in enzymatic activity in alkaline pH values | Schistocerca gregaria |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000189 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | Cibacron blue | |
0.00223 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | quercetin | |
0.00388 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | delphinidin chloride | |
0.0103 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | rutin | |
0.363 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | bromosulfophthalein | |
3.63 | - |
at pH 8.0 and 30°C | Schistocerca gregaria | Ethacrynic acid |