Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
E68A | the mutant retains activity toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole, 1-chloro-2,4-dinitrobenzene and 4-nitrobenzyl chloride, but its activities drop significantly compared to the wild type enzyme | Oryza sativa |
F116A | the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
F162A | the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
K42A | the mutant shows 10% of wild type activity | Oryza sativa |
L112A | the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
M17A | the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
N109A | the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
S15A | inactive | Oryza sativa |
S69A | the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 31% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme | Oryza sativa |
V56A | the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 21% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme | Oryza sativa |
W161A | the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
Y113A | the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
glutathione | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa | |
0.12 | - |
glutathione | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.166 | - |
glutathione | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.192 | - |
glutathione | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.221 | - |
glutathione | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.307 | - |
glutathione | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.324 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa | |
0.36 | - |
glutathione | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.47 | 1 | 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.743 | - |
glutathione | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.781 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.882 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.117 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.487 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa | |
3.661 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
3.866 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q93WY5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Oryza sativa | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole | - |
Oryza sativa | ? | - |
? | |
glutathione + 4-nitrobenzyl chloride | - |
Oryza sativa | chloride + 4-nitrobenzyl-glutathione | - |
? | |
glutathione + cumene hydroperoxide | - |
Oryza sativa | ? | - |
? | |
additional information | no activity toward 1,2-dichloro-4-nitrobenzene, 4-nitrophenyl acetate and ethacrynic acid | Oryza sativa | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Oryza sativa |
GSTU17 | isoform | Oryza sativa |
tau class glutathione S-transferase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | the enzyme retains about 85, 99, 95 and 65% activity after 15 min at 25, 35, 45 and 55°C, respectively. The enzyme is inactivated at 65°C | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.196 | - |
glutathione | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.22 | - |
glutathione | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.257 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa | |
0.264 | - |
glutathione | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa | |
0.266 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.277 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.322 | - |
glutathione | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.387 | - |
glutathione | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.421 | - |
glutathione | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.512 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.573 | - |
glutathione | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.809 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.946 | - |
glutathione | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.999 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.573 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.75 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.221 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.341 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.407 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.521 | - |
glutathione | mutant enzyme M17A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.581 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.589 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
0.793 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa | |
0.894 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.019 | - |
glutathione | mutant enzyme N109A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.17 | - |
glutathione | mutant enzyme F162A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.177 | - |
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.579 | - |
glutathione | mutant enzyme L112A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.842 | - |
glutathione | mutant enzyme Y113A, at 25°C, pH not specified in the publication | Oryza sativa | |
1.942 | - |
glutathione | mutant enzyme F116A, at 25°C, pH not specified in the publication | Oryza sativa | |
2.588 | - |
glutathione | mutant enzyme W161A, at 25°C, pH not specified in the publication | Oryza sativa | |
4.552 | - |
glutathione | wild type enzyme, at 25°C, pH not specified in the publication | Oryza sativa |