Literature summary for 2.5.1.18 extracted from

Al-Madboly, L.A.; Ali, S.M.; Fakharany, E.M.E.; Ragab, A.E.; Khedr, E.G.; Elokely, K.M.
Stress-based production, and characterization of glutathione peroxidase and glutathione S-transferase enzymes from Lactobacillus plantarum (2020), Front. Bioeng. Biotechnol., 8, 78 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	98.7% residual activity at 5 mM	Lactiplantibacillus plantarum
dithiothreitol	32% residual activity at 10 mM	Lactiplantibacillus plantarum
EDTA	90% residual activity at 10 mM	Lactiplantibacillus plantarum
Fe2+	40% residual activity at 5 mM	Lactiplantibacillus plantarum
Mg2+	89.3% residual activity at 5 mM	Lactiplantibacillus plantarum
SDS	61% residual activity at 10 mM	Lactiplantibacillus plantarum
Zn2+	65.4% residual activity at 5 mM	Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	5 mM Mn2+ increases the enzyme activity slightly to 101%	Lactiplantibacillus plantarum
additional information	not influenced by Ca2+, K+, and Na+	Lactiplantibacillus plantarum

Organism

Organism	UniProt	Comment	Textmining
Lactiplantibacillus plantarum	-	-	-
Lactiplantibacillus plantarum KU720558	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and GSH-agarose affinity chromatography	Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1789.5	-	crude extract, at pH 6.0 and 40°C	Lactiplantibacillus plantarum
3980.6	-	after 2.22fold purification, at pH 6.0 and 40°C	Lactiplantibacillus plantarum

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE	Lactiplantibacillus plantarum

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Lactiplantibacillus plantarum
GST	-	Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	60	the enzyme retains 70% of its activity at 50°C and lose about 36% of the activity at 60°C. A marked decrease in the relative activity of the enzyme of 25% is noticed at 70°C	Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	70	the enzyme retains its full activity for 1 h at 4-40°C. After 1 h at 50, 60, and 70°C, the enzyme retains about 80, 60 and 10% of its activity, respectively	Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Lactiplantibacillus plantarum

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7	the enzyme is able to retain its activity at pH range 4.0-7.0. Slight reduction in the activity is recorded at pH 8.0	Lactiplantibacillus plantarum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	7	the enzyme retains its activity for 1 h at the pH range 4.0-7.0	Lactiplantibacillus plantarum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Lactiplantibacillus plantarum	isoelectric focusing	-	7

Expression

Organism	Comment	Expression
Lactiplantibacillus plantarum	0.05% (v/v) H2O2, 0.05% (w/v) SDS, 0.1% (w/v) urea, 1% (v/v) 1-butanol, 0.05% (2/v) bile salt, 2% (v/v) ethanol, 0.05% (w/v) equally mixed amino acids, pH 6.0 and anaerobic incubation at 40°C for 24 h enhances enzyme productivity	up

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Release 2023.1 (January 2023)