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Literature summary for 2.5.1.18 extracted from
- New substrates and activity of Phanerochaete chrysosporium Omega glutathione transferases (2013), Biochimie, 95, 336-346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Phanerodontia chrysosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C18S | the mutant of isoform GSTO4 exhibits a similar pattern of activity than the wild type protein | Phanerodontia chrysosporium |
| C18S | the mutant of isoform GSTO7 exhibits a similar pattern of activity than the wild type protein | Phanerodontia chrysosporium |
| C37S | inactive mutant of isoform GSTO3 | Phanerodontia chrysosporium |
| C37S | inactive mutant of isoform GSTO4 | Phanerodontia chrysosporium |
| C91S | the mutant of isoform GSTO4 exhibits a similar pattern of activity than the wild type protein | Phanerodontia chrysosporium |
| C91S | the mutant of isoform GSTO7 exhibits a similar pattern of activity than the wild type protein | Phanerodontia chrysosporium |
| S15C | the inactive mutant of isoform GSTO4 is able to bind covalently a glutathione | Phanerodontia chrysosporium |
| S15C | the inactive mutant of isoform GSTO7 is able to bind covalently a glutathione | Phanerodontia chrysosporium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-bromo-1-tetralone | - |
Phanerodontia chrysosporium |  |
| camphene | - |
Phanerodontia chrysosporium | |
| carene | - |
Phanerodontia chrysosporium | |
| caryophyllene | - |
Phanerodontia chrysosporium | |
| caryophyllene oxide | - |
Phanerodontia chrysosporium | |
| gallic acid | - |
Phanerodontia chrysosporium | |
| limonene | - |
Phanerodontia chrysosporium | |
| longifolene | - |
Phanerodontia chrysosporium | |
| menadione | - |
Phanerodontia chrysosporium | |
| myrcene | - |
Phanerodontia chrysosporium | |
| Pentachlorophenol | - |
Phanerodontia chrysosporium | |
| phenylacetophenone | - |
Phanerodontia chrysosporium | |
| pinene | - |
Phanerodontia chrysosporium | |
| rifampicin | - |
Phanerodontia chrysosporium | |
| S-glutathionyl-phenylacetophenone | - |
Phanerodontia chrysosporium | |
| S-glutationyl-thiodione | - |
Phanerodontia chrysosporium | |
| S-phenylacetylglutathione | - |
Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
phenethyl isothiocyanate | isoform GSTO7, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.207 | - |
S-phenylacetylglutathione | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 1.222 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTO7, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 1.66 | - |
dehydroascorbate | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26938 | - |
- |
Phanerodontia chrysosporium |
| 30019 | - |
- |
Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
isoform GSTO4 | - |
| Phanerodontia chrysosporium | B3VQJ6 | isoform GSTO3 | - |
| Phanerodontia chrysosporium | J7JYU5 | isoform GSTO7 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chloro-2,4-dinitrobenzene + glutathione | - |
Phanerodontia chrysosporium | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| dehydroascorbate + glutathione | - |
Phanerodontia chrysosporium | ? | - |
? | |
| additional information | isoform GSTO7 shows no activity with hydroxyethyldisulfide, dehydroascorbate, and S-glutathionyl-phenylacetophenone | Phanerodontia chrysosporium | ? | - |
? | |
| phenethyl isothiocyanate + glutathione | - |
Phanerodontia chrysosporium | ? | - |
? | |
| S-glutathionyl-phenylacetophenone + glutathione | - |
Phanerodontia chrysosporium | ? | - |
? | |
| S-glutathionyltetralone + glutathione | - |
Phanerodontia chrysosporium | ? | - |
? | |
| S-phenylacetylglutathione + glutathione | - |
Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26938, isoform GSTO7, calculated from amino acid sequence | Phanerodontia chrysosporium |
| ? | x * 26938, native isoform GSTO7, mass spectrometry | Phanerodontia chrysosporium |
| ? | x * 27713, isoform GSTO4, calculated from amino acid sequence | Phanerodontia chrysosporium |
| ? | x * 30019, native isoform GSTO4, mass spectrometry | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GSTO3 | isoform | Phanerodontia chrysosporium |
| GSTO4 | isoform | Phanerodontia chrysosporium |
| GSTO7 | isoform | Phanerodontia chrysosporium |
| omega glutathione transferase | - |
Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.26 | - |
dehydroascorbate | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 1.69 | - |
phenethyl isothiocyanate | isoform GSTO7, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 2.08 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTO7, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 180 | - |
S-phenylacetylglutathione | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00061 | - |
S-glutathionyl-phenylacetophenone | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.00087 | - |
S-phenylacetylglutathione | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.001691 | - |
phenylacetophenone | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.003148 | - |
S-glutationyl-thiodione | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.003168 | - |
phenylacetophenone | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.004542 | - |
S-glutationyl-thiodione | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.01444 | - |
Pentachlorophenol | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.0161 | - |
2-bromo-1-tetralone | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.01632 | - |
2-bromo-1-tetralone | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.02111 | - |
rifampicin | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.02498 | - |
menadione | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.03591 | - |
menadione | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.04558 | - |
rifampicin | isoform GSTO4, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
| 0.06904 | - |
Pentachlorophenol | isoform GSTO3, at pH 6.5 and 20°C | Phanerodontia chrysosporium | |
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