Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant GSTP1-1s in Escherichia coli | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant GSTP1-1 C47A mutant, hanging drop vapour diffusion method, mixing of 0.002 ml of 7 mg/ml protein in 10 mM MES, pH 6.0, with 0.0006 ml of 30 mM S-(p-nitrobenzyl)glutathione in 50 mM MES, pH 6.0, and 0.003 ml of reservoir solution containing 20% PEG 6000 and 0.1 M sodium citrate, pH 4.0, several weeks, 20°C, X-ray diffraction structure determmintion and analysis at 1.77 A resolution | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C14A | site-directed mutagenesis | Mus musculus |
C14A/C169A | site-directed mutagenesis | Mus musculus |
C14A/C47A | site-directed mutagenesis | Mus musculus |
C169A | site-directed mutagenesis | Mus musculus |
C47A | site-directed mutagenesis | Mus musculus |
C47A/C169A | site-directed mutagenesis | Mus musculus |
additional information | the native enzyme and the C169A mutant exhibit Michaelis-Menten kinetics, but all other thiol to alanine mutants exhibit sigmoidal kinetics to varying degrees | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-nitrobenzylglutathione | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the native enzyme and the C169A mutant exhibit MichaelisMenten kinetics, but all other thiol to alanine mutants exhibit sigmoidal kinetics to varying degrees, ping-pong kinetics of mutant C169A, overview | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P19157 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GSTP1-1s from Escherichia coli by glutathione affinity chromatography | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | the mutant C169A exhibits cooperative behaviour and a ping pong mechanism whereby liberation of a proton from a reduced enzyme-glutathione complex to form an enzyme-unprotonated glutathione complex is essentially irreversible | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
glutathione transferase P1-1 | - |
Mus musculus |
GSTP1-1 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Mus musculus |