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Literature summary for 2.5.1.18 extracted from

  • Dixon, D.P.; Sellars, J.D.; Edwards, R.
    The Arabidopsis phi class glutathione transferase AtGSTF2: binding and regulation by biologically active heterocyclic ligands (2011), Biochem. J., 438, 63-70.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally Strep-tagged AtGSTF2 and AtGSTF3 in Escherichia coli, expression of untagged GSTF2 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information isothermal titration calorimetry reveals high-affinity binding for GSTF2 and GSTF3, which is enhanced in the presence of glutathione and by the other heterocyclic ligands, and allosteric enhancement in glutathione-conjugating activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane GSTF2 association with membrane vesicles Arabidopsis thaliana 16020
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Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally Strep-tagged AtGSTF2 and AtGSTF3 from Escherichia coli by affinity chromatography and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information plant-specific phi class of glutathione transferases, GSTFs, are often highly stress-inducible and expressed in a tissue-specific manner Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information overexpressed in bacteria, family members GSTF2 and GSTF3 bind a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. GSTF2 also selectively binds the indole-derived phytoalexin camalexin, as well as the flavonol quercetin-3-O-rhamnoside, recombinant GSTF2 ligand binding studies and kinetics, overview Arabidopsis thaliana ?
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?

Synonyms

Synonyms Comment Organism
AtGSTF2
-
Arabidopsis thaliana
phi class glutathione transferase
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Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana plant-specific phi class of glutathione transferases, GSTFs, are often highly stress-inducible and expressed in a tissue-specific manner up

General Information

General Information Comment Organism
physiological function GSTF2 and GSTF3 may have roles in regulating the binding and transport of defence-related compounds in planta Arabidopsis thaliana