Literature summary for 2.5.1.18 extracted from

Salazar-Medina, A.J.; Garcia-Rico, L.; Garcia-Orozco, K.D.; Valenzuela-Soto, E.; Contreras-Vergara, C.A.; Arreola, R.; Arvizu-Flores, A.; Sotelo-Mundo, R.R.
Inhibition by Cu2+ and Cd2+ of a mu-class glutathione S-transferase from shrimp Litopenaeus vannamei (2010), J. Biochem. Mol. Toxicol., 24, 218-222.

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Cloned(Commentary)

Cloned (Comment)	Organism
a synthetic GST gene based on the wild type amino acid sequence, changing the second residue to an isoleucine, is overexpressed in Escherichia coli strain BL21 to obtain GST as a soluble protein	Penaeus vannamei

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	binds in the GSH-binding site by coordination with Asp and Gln residues, molecular modeling, overview	Penaeus vannamei
Cu2+	-	Penaeus vannamei

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the residues that coordinate the metal ion in the shrimp GST are N59, Q72 from one monomer and D106 from the partner chain	Penaeus vannamei

Organism

Organism	UniProt	Comment	Textmining
Penaeus vannamei	Q49SB0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Ile2 enzyme variant from Escherichia coli strain BL21 by glutathione affinity chromatography	Penaeus vannamei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Penaeus vannamei	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Penaeus vannamei
GST	-	Penaeus vannamei
mu class glutathione S-transferase	-	Penaeus vannamei

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Penaeus vannamei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Penaeus vannamei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00039	-	-	Penaeus vannamei	Cd2+
0.00477	-	-	Penaeus vannamei	Cu2+

General Information

General Information	Comment	Organism
physiological function	glutathione S-transferases are a family of detoxifying enzymes that catalyze the conjugation of glutathione to electrophiles, thereby increasing the solubility of xenobiotics and aiding its excretion from the cell	Penaeus vannamei

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Release 2023.1 (January 2023)