Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant R15L isozyme GSTA1-1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
recombinant mutant R15L isozyme GSTA1-1 complexed with inhibitor S-hexylglutathione, hanging drop vapor diffusion method, 0.002 ml of 14 mg/ml R15L GSTA1-1 in 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, 0.02% sodium azide solution are mixed with 0.002 ml of reservoir buffer containing 5 mM S-hexylglutathione, 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, 5-30% PEG 2000 or 4000, equilibration against 1 ml reservoir solution, 3 days, X-ray diffraction structure determination and analysis at 1.80 A resolution, molecular replacement method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R15L | site-directed mutagenesis, the mutation substantially diminishes the 1-chloro-2,4-dinitrobenzene-GSH conjugating activity of the enzyme, it has little effect on protein structure and stability | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-hexylglutathione | binding structure, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic and molecular docking of wild-type and R15L mutant enzymes, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08263 | isozyme GSTA1-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant R15L isozyme GSTA1-1 from Escherichia coli strain BL21(DE3) by ion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,5-trinitrobenzene + glutathione | an anionic sigma-complex is formed between GSH and 1,3,5-trinitrobenzene and is stabilized by Arg15. The trinitrocyclohexadienate moiety of the sigma-complex binds the H-site where the catalytic residue, Tyr9, was identified to hydrogen bond to an o-nitro group of the sigma-complex | Homo sapiens | S-(2,4,6-trinitrocyclohexa-2,5-dien-1-yl)glutathione | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | SNAr reaction between GSH and 1-chloro-2,4-dinitrobenzene. Role of Arg15 in the binding and activation of GSH and in sigma-complex formation, overview | Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | Arg15, conserved in class alpha GSTs, is located at the interface between the G- and H-sites of the active site where its cationic guanidinium group might play a role in catalysis and ligand binding. Binding of the anionic non-substrate ligand 8-anilino-1-naphthalene sulfonate to the H-site involving residue Arg15. The sigma-complex 5-(2,4,6-trinitrocyclohexa-2,5-dien-1-yl)glutathione is an analogue of the anionic transition state of the SNAr reaction between S-(2,4-dinitrophenyl)glutathione and GSH, docking structure, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha GST | - |
Homo sapiens |
glutathione transferase A1-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |